兔多克隆抗体to hnRNP M1-M4
Synthetic peptide derived from the N-terminal region of human hnRNP M1-M4.
WB: extracts from HT29 cells;
IHC-P: human breast carcinoma tissue;
ICC/IF: MCF7 cells.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg 2+ and Ca 2+), 150mM Sodium chloride, pH 7.4
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Predicted molecular weight: 78 kDa.
1/50 - 1/100.
1/500 - 1/1000.
Pre-mRNA binding protein in vivo, binds avidly to poly(G) and poly(U) RNA homopolymers in vitro. Involved in splicing. Acts as a receptor for carcinoembryonic antigen in Kupffer cells, may initiate a series of signaling events leading to tyrosine phosphorylation of proteins and induction of IL-1 alpha, IL-6, IL-10 and tumor necrosis factor alpha cytokines.
Contains 3 RRM (RNA recognition motif) domains.
Nucleus > nucleolus.
Information by UniProt
CEA receptor antibody
Anti-hnRNP M1-M4 antibody 图像
Western blot - hnRNP M1-M4 antibody (ab73888)
All lanes :
Anti-hnRNP M1-M4 antibody (ab73888) at 1/500 dilution
Lane 1 :
extracts from HT29 cells
Lane 2 :
extracts from HT29 cells with immunizing peptide at 5 µg
Lysates/proteins at 20 µg per lane.
Predicted band size :
Observed band size :
85 kDa (
why is the actual band size different from the predicted?
Immunocytochemistry/ Immunofluorescence - hnRNP M1-M4 antibody (ab73888)
Left: immunofluorescence analysis of MCF7 cells, using 1/500-1/1000 ab73888; Right: same after pre-incubation with the immunizing peptide.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - hnRNP M1-M4 antibody (ab73888)
Left: immunohistochemistry analysis of paraffin-embedded
human breast carcinoma tissue using 1/50-1/100 ab73888; Right: same, after per-incubation with the immunizing peptide.
Anti-hnRNP M1-M4 antibody (ab73888)参考文献
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"