概述

  • 产品名称Anti-hnRNP L抗体
    参阅全部 hnRNP L 一抗
  • 描述
    兔多克隆抗体to hnRNP L
  • 经测试应用适用于: ELISA, ICC/IF, IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide from N terminal of human hnRNP L.

  • 阳性对照
    • Human colon carcinoma tissue, HeLa, Jurkat and HUVEC cell extracts and HeLa cells.

性能

应用

Our Abpromise guarantee covers the use of ab65049 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/20000.
ICC/IF 1/500 - 1/1000.
IHC-P 1/50 - 1/100.
WB 1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).

靶标

  • 功能This protein is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Is associated with most nascent transcripts including those of the landmark giant loops of amphibian lampbrush chromosomes. Associates, together with APEX1, to the negative calcium responsive element (nCaRE) B2 of the APEX2 promoter.
  • 序列相似性Contains 3 RRM (RNA recognition motif) domains.
  • 翻译后修饰Several isoelectric forms of the L protein are probably the results of post-translational modifications.
  • 细胞定位Nucleus > nucleoplasm. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Information by UniProt
  • 数据库链接
  • 别名
    • D830027H13Rik antibody
    • FLJ35509 antibody
    • Heterogeneous nuclear ribonucleoprotein L antibody
    • hnRNP L antibody
    • hnRNP-L antibody
    • Hnrnpl antibody
    • hnRPL antibody
    • HNRPL_HUMAN antibody
    • P/OKcl.14 antibody
    see all

Anti-hnRNP L antibody 图像

  • ab65049 at 1/500 dilution staining hnRNP L in HeLa cells by Immunofluorescence, in the absence or presence of the immunising peptide.
  • ab65049 at 1/50 dilution staining hnRNP L in human colon carcinoma by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide.
  • All lanes : Anti-hnRNP L antibody (ab65049) at 1/500 dilution

    Lane 1 : HeLa cell extract
    Lane 2 : Jurkat cell extract
    Lane 3 : HUVEC cell extract
    Lane 4 : HUVEC cell extract with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 60 kDa
    Observed band size : 60 kDa

Anti-hnRNP L antibody (ab65049)参考文献

This product has been referenced in:
  • Roda D  et al. EGF-Induced Acetylation of Heterogeneous Nuclear Ribonucleoproteins Is Dependent on KRAS Mutational Status in Colorectal Cancer Cells. PLoS One 10:e0130543 (2015). Human . Read more (PubMed: 26110767) »
  • Li J  et al. HnRNPL as a key factor in spermatogenesis: Lesson from functional proteomic studies of azoospermia patients with sertoli cell only syndrome. J Proteomics : (2012). WB ; Human . Read more (PubMed: 22245417) »

See all 4 Publications for this product

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