The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000.
1/50 - 1/100.
1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
功能This protein is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Is associated with most nascent transcripts including those of the landmark giant loops of amphibian lampbrush chromosomes. Associates, together with APEX1, to the negative calcium responsive element (nCaRE) B2 of the APEX2 promoter.
Heterogeneous nuclear ribonucleoprotein L antibody
hnRNP L antibody
Anti-hnRNP L antibody 图像
Immunocytochemistry/ Immunofluorescence - hnRNP L antibody (ab65049)
ab65049 at 1/500 dilution staining hnRNP L in HeLa cells by Immunofluorescence, in the absence or presence of the immunising peptide.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - hnRNP L antibody (ab65049)
ab65049 at 1/50 dilution staining hnRNP L in human colon carcinoma by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide.
Western blot - hnRNP L antibody (ab65049)
All lanes : Anti-hnRNP L antibody (ab65049) at 1/500 dilution
Lane 1 : HeLa cell extract Lane 2 : Jurkat cell extract Lane 3 : HUVEC cell extract Lane 4 : HUVEC cell extract with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size : 60 kDa Observed band size : 60 kDa
Anti-hnRNP L antibody (ab65049)参考文献
This product has been referenced in:
Roda D et al. EGF-Induced Acetylation of Heterogeneous Nuclear Ribonucleoproteins Is Dependent on KRAS Mutational Status in Colorectal Cancer Cells. PLoS One10:e0130543 (2015).
Read more (PubMed: 26110767) »
Li J et al. HnRNPL as a key factor in spermatogenesis: Lesson from functional proteomic studies of azoospermia patients with sertoli cell only syndrome. J Proteomics : (2012).
Read more (PubMed: 22245417) »