概述

  • 产品名称Anti-hnRNP L抗体
    参阅全部 hnRNP L 一抗
  • 描述
    兔多克隆抗体to hnRNP L
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Xenopus laevis
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human hnRNP L.

    (Peptide available as ab45316.)

  • 阳性对照
    • This antibody gave a positive signal in the following whole cell lysates: HeLa (Human epithelial carcinoma cell line); Jurkat (Human T cell lymphoblast-like cell line); A431 (Human epithelial carcinoma cell line); HEK293 (Human embryonic kidney cell line); MCF7 (Human breast adenocarcinoma cell line); HepG2 (Human hepatocellular liver carcinoma cell line); SHSY-5Y (Human neuroblastoma cell line)

性能

应用

Our Abpromise guarantee covers the use of ab45317 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 60 kDa).
ICC/IF Use a concentration of 1 µg/ml.

靶标

  • 功能This protein is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Is associated with most nascent transcripts including those of the landmark giant loops of amphibian lampbrush chromosomes. Associates, together with APEX1, to the negative calcium responsive element (nCaRE) B2 of the APEX2 promoter.
  • 序列相似性Contains 3 RRM (RNA recognition motif) domains.
  • 翻译后修饰Several isoelectric forms of the L protein are probably the results of post-translational modifications.
  • 细胞定位Nucleus > nucleoplasm. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Information by UniProt
  • 数据库链接
  • 别名
    • D830027H13Rik antibody
    • FLJ35509 antibody
    • Heterogeneous nuclear ribonucleoprotein L antibody
    • hnRNP L antibody
    • hnRNP-L antibody
    • Hnrnpl antibody
    • hnRPL antibody
    • HNRPL_HUMAN antibody
    • P/OKcl.14 antibody
    see all

Anti-hnRNP L antibody 图像

  • All lanes : Anti-hnRNP L antibody (ab45317) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 4 : HEK293 Human embryonic kidney cell line Whole Cell Lysate
    Lane 5 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 6 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 7 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 60 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 85 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab45317 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in PBS-T (20 min) and incubated with the antibody (ab45317, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

Anti-hnRNP L antibody (ab45317)参考文献

ab45317 has not yet been referenced specifically in any publications.

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