Anti-hnRNP L抗体(ab156682)
Key features and details
- Rabbit polyclonal to hnRNP L
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-hnRNP L抗体
参阅全部 hnRNP L 一抗 -
描述
兔多克隆抗体to hnRNP L -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Chimpanzee, Ferret, Non human primates, Rhesus monkey, Gorilla, Orangutan -
免疫原
Synthetic peptide corresponding to a region within amino acids 350-400 of Human hnRNP L (NP_001524.2).
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阳性对照
- 293T, HeLa and Jurkat whole cell lysates; mouse NIH 3T3 whole cell lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab156682 was affinity purified using an epitope specific to hnRNP L immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab156682于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 64 kDa.
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IP |
Use at 2-10 µg/mg of lysate.
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说明 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 64 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
靶标
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功能
This protein is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Is associated with most nascent transcripts including those of the landmark giant loops of amphibian lampbrush chromosomes. Associates, together with APEX1, to the negative calcium responsive element (nCaRE) B2 of the APEX2 promoter. -
序列相似性
Contains 3 RRM (RNA recognition motif) domains. -
翻译后修饰
Several isoelectric forms of the L protein are probably the results of post-translational modifications. -
细胞定位
Nucleus > nucleoplasm. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. - Information by UniProt
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数据库链接
- Entrez Gene: 504520 Cow
- Entrez Gene: 476466 Dog
- Entrez Gene: 100734238 Guinea pig
- Entrez Gene: 100066483 Horse
- Entrez Gene: 3191 Human
- Entrez Gene: 15388 Mouse
- Entrez Gene: 80846 Rat
- Omim: 603083 Human
see all -
别名
- D830027H13Rik antibody
- FLJ35509 antibody
- Heterogeneous nuclear ribonucleoprotein L antibody
see all
图片
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All lanes : Anti-hnRNP L antibody (ab156682) at 0.1 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg
Lane 2 : 293T whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 64 kDa
Exposure time: 30 seconds -
All lanes : Anti-hnRNP L antibody (ab156682) at 0.4 µg/ml
Lane 1 : Mouse NIH 3T3 whole cell lysate at 50 µg
Lane 2 : Mouse NIH 3T3 whole cell lysate at 15 µg
Developed using the ECL technique.
Predicted band size: 64 kDa
Exposure time: 10 seconds -
Detection of hnRNP L by Western blot of Immunoprecipitate
Lane 1: Immunoprecipitation of 293T whole cell lysate using ab156682 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2: lysate is precipitated with an antibody to another region of hnRNP L.
Lane 3: lysate is precipitated using an IgG control antibody.
Detection: using ab156682 at 1 µg/ml.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab156682 被引用在 1 文献中.
- Cera I et al. Genes encoding SATB2-interacting proteins in adult cerebral cortex contribute to human cognitive ability. PLoS Genet 15:e1007890 (2019). PubMed: 30726206