Anti-hnRNP K (phospho S284)抗体(ab74066)

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概述

  • 产品名称
    Anti-hnRNP K (phospho S284)抗体
    参阅全部 hnRNP K 一抗
  • 描述
    兔多克隆抗体to hnRNP K (phospho S284)
  • 特异性
    ab74066 detects endogenous levels of hnRNP K only when phosphorylated at serine 284.
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic phosphopeptide derived from human hnRNP K around the phosphorylation site of serine 284 (D-M-SP-P-R).

  • 阳性对照
    • Extracts from 3T3 cells treated with EGF (200ng/ml, 30mins).

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    ab74066 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab74066 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).
ELISA 1/20000.

靶标

  • 功能
    One of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single-stranded DNA.
  • 序列相似性
    Contains 3 KH domains.
  • 翻译后修饰
    Arg-296 and Arg-299 are dimethylated, probably to asymmetric dimethylarginine.
  • 细胞定位
    Cytoplasm. Nucleus > nucleoplasm. In case of ASFV infection, there is a shift in the localization which becomes predominantly nuclear.
  • Information by UniProt
  • 数据库链接
    • 别名
      • CSBP antibody
      • dC stretch binding protein antibody
      • FLJ41122 antibody
      • Heterogeneous nuclear ribonucleoprotein K antibody
      • hnRNP K antibody
      • HNRNPK antibody
      • HNRPK antibody
      • HNRPK_HUMAN antibody
      • Transformation up regulated nuclear protein antibody
      • Transformation up-regulated nuclear protein antibody
      • Transformation upregulated nuclear protein antibody
      • TUNP antibody
      see all

    Anti-hnRNP K (phospho S284) antibody 图像

    • Western blot - hnRNP K (phospho S284) antibody (ab74066)
      Western blot - hnRNP K (phospho S284) antibody (ab74066)
      All lanes : Anti-hnRNP K (phospho S284) antibody (ab74066) at 1/500 dilution

      Lane 1 : Extracts from 3T3 cells treated with EGF (200ng/ml, 30mins)
      Lane 2 : Extracts from 3T3 cells treated with EGF (200ng/ml, 30mins) with immunising phosphopeptide at 10 µg

      Lysates/proteins at 30 µg per lane.


      Predicted band size : 51 kDa
      Observed band size : 51 kDa

    实验方案

    Anti-hnRNP K (phospho S284) antibody (ab74066)参考文献

    ab74066 has not yet been referenced specifically in any publications.

    发表研究结果有使用 ab74066？请让我们知道，以便我们可以引用本数据表中的参考文章。

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    A customer has just called and would like to know if you have made a blocking protein for this antibody?

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    Unfortunately the blocking peptide for this antibody is not available.

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