Anti-hnRNP K (phospho S284)抗体(ab74066)

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概述

  • 产品名称
    Anti-hnRNP K (phospho S284)抗体
    参阅全部 hnRNP K 一抗
  • 描述
    兔多克隆抗体to hnRNP K (phospho S284)
  • 宿主
    Rabbit
  • 特异性
    ab74066 detects endogenous levels of hnRNP K only when phosphorylated at serine 284.
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic phosphopeptide derived from human hnRNP K around the phosphorylation site of serine 284 (D-M-SP-P-R).

  • 阳性对照
    • Extracts from 3T3 cells treated with EGF (200ng/ml, 30mins).

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    ab74066 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab74066 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).
ELISA 1/20000.

靶标

  • 功能
    One of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single-stranded DNA.
  • 序列相似性
    Contains 3 KH domains.
  • 翻译后修饰
    Arg-296 and Arg-299 are dimethylated, probably to asymmetric dimethylarginine.
  • 细胞定位
    Cytoplasm. Nucleus > nucleoplasm. In case of ASFV infection, there is a shift in the localization which becomes predominantly nuclear.
  • Information by UniProt
  • 数据库链接
    • 别名
      • CSBP antibody
      • dC stretch binding protein antibody
      • FLJ41122 antibody
      • Heterogeneous nuclear ribonucleoprotein K antibody
      • hnRNP K antibody
      • HNRNPK antibody
      • HNRPK antibody
      • HNRPK_HUMAN antibody
      • Transformation up regulated nuclear protein antibody
      • Transformation up-regulated nuclear protein antibody
      • Transformation upregulated nuclear protein antibody
      • TUNP antibody
      see all

    图片

    • Western blot - Anti-hnRNP K (phospho S284) antibody (ab74066)
      Western blot - Anti-hnRNP K (phospho S284) antibody (ab74066)
      All lanes : Anti-hnRNP K (phospho S284) antibody (ab74066) at 1/500 dilution

      Lane 1 : Extracts from 3T3 cells treated
      with EGF (200ng/ml, 30mins)
      Lane 2 : Extracts from 3T3 cells treated
      with EGF (200ng/ml, 30mins) with immunising phosphopeptide at 10 µg

      Lysates/proteins at 30 µg per lane.

      Predicted band size: 51 kDa
      Observed band size: 51 kDa

    实验方案

    文献

    ab74066 has not yet been referenced specifically in any publications.

    发表研究结果有使用 ab74066？请让我们知道，以便我们可以引用本数据表中的参考文章。

    客户评价及客户问答

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    1 Review or Q&A

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    A customer has just called and would like to know if you have made a blocking protein for this antibody?

    Thanks for your help and hope to hear from you soon!

    Kind regards,

    Thank you for contacting us.

    Unfortunately the blocking peptide for this antibody is not available.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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