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A synthetic peptide corresponding to residues near the N-terminus of human hnRNP.
This product is a recombinant rabbit monoclonal antibody.
Alternative versions available:
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
Our Abpromise guarantee covers the use of ab52600 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100000. Detects a band of approximately 60 kDa (predicted molecular weight: 51 kDa).|
|IHC-P||Use at an assay dependent concentration.|
|ICC/IF||1/100 - 1/250.|
ab52600 at 1/100 immunoprecipitating hnRNP K in HeLa whole cell lysate.
For western blotting, a primary antibody was used at 1/1000 and a HRP-conjugated goat-anti-rabbit IgG was used as the secondary antibody (1/1000).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"