概述

  • 产品名称Anti-hnRNP K抗体- ChIP Grade
    参阅全部 hnRNP K 一抗
  • 描述
    兔多克隆抗体to hnRNP K - ChIP Grade
  • 特异性This antibody does not appear to react with isoform b of human hnRNPK (NP_112552.1).
  • 经测试应用适用于: WB, IP, IHC-P, ICC/IF, ChIP/Chipmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Guinea pig, Cow, Rhesus monkey, Gorilla, Orangutan, Platypus (Ornithorhynchus anatinus), Elephant
  • 免疫原

    Synthetic peptide corresponding to a sequence from the C-terminus of isoform a of human hnRNP K. (NP_002131.2)

  • 阳性对照
    • Whole cell lysate from HeLa, 293T and mouse NIH3T3 cells. IF//ICC: HeLa cell line. This antibody gave a positive result in IHC in the following FFPE tissue: Human Colon Adenocarcinoma.

性能

应用

Our Abpromise guarantee covers the use of ab70492 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 51 kDa).
IP Use at 1-4 µg/mg of lysate.
IHC-P Use a concentration of 0.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 µg/ml.
ChIP/Chip Use at an assay dependent concentration. PubMed: 20673990

靶标

  • 功能One of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single-stranded DNA.
  • 序列相似性Contains 3 KH domains.
  • 翻译后修饰Arg-296 and Arg-299 are dimethylated, probably to asymmetric dimethylarginine.
  • 细胞定位Cytoplasm. Nucleus > nucleoplasm. In case of ASFV infection, there is a shift in the localization which becomes predominantly nuclear.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CSBP antibody
    • dC stretch binding protein antibody
    • FLJ41122 antibody
    • Heterogeneous nuclear ribonucleoprotein K antibody
    • hnRNP K antibody
    • HNRNPK antibody
    • HNRPK antibody
    • HNRPK_HUMAN antibody
    • Transformation up regulated nuclear protein antibody
    • Transformation up-regulated nuclear protein antibody
    • Transformation upregulated nuclear protein antibody
    • TUNP antibody
    see all

Anti-hnRNP K antibody - ChIP Grade 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma (left) and mouse squamous cell carcinoma (right) tissues labelling hnRNP K with ab70492 at 1/1000 (0.2µg/ml). Detection: DAB.
  • IHC image of hnRNP K staining in Human Colon Adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab70492, 0.5 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ICC/IF image of ab70492 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70492, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-hnRNP K antibody - ChIP Grade (ab70492) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg


    Predicted band size : 51 kDa
    Observed band size : 51,55 kDa (why is the actual band size different from the predicted?)
  • Detection of Human hnRNP K by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg/IP, 20% of IP loaded) using ab70492 at 3 µg/mg for IP and at 1.0 µg/ml for subsequent WB detection. Lane 2 represents IP IgG control.

Anti-hnRNP K antibody - ChIP Grade (ab70492)参考文献

This product has been referenced in:
  • Thompson PJ  et al. hnRNP K Coordinates Transcriptional Silencing by SETDB1 in Embryonic Stem Cells. PLoS Genet 11:e1004933 (2015). ChIP, ICC/IF . Read more (PubMed: 25611934) »
  • Dimitrova N  et al. LincRNA-p21 activates p21 in cis to promote Polycomb target gene expression and to enforce the G1/S checkpoint. Mol Cell 54:777-90 (2014). WB, ChIP . Read more (PubMed: 24857549) »

See all 3 Publications for this product

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