The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. PubMed: 22436695
1/500 - 1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
1/500 - 1/1000.
相关性Heterogeneous nuclear ribonuclearproteins (hnRNPs) are nucleic acid binding proteins and have critical roles in DNA repair, telomere regulation, and transcriptional gene regulation. hnRNP G has tumor suppressive activity and this RNA binding protein which may be involved in pre-mRNA splicing.
Heterogeneous nuclear ribonucleoprotein G antibody
hnRNP G antibody
RNA binding motif protein, X chromosome antibody
RNA binding motif protein, X linked antibody
Anti-hnRNP G antibody 图像
Western blot - hnRNP G antibody (ab70064)
All lanes : Anti-hnRNP G antibody (ab70064) at 1/500 dilution
Lane 1 : Extracts from HeLa cells Lane 2 : Extracts from HeLa cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size : 42 kDa Observed band size : 42 kDa Additional bands at : 80 kDa. We are unsure as to the identity of these extra bands.
Immunocytochemistry/ Immunofluorescence - hnRNP G antibody (ab70064)
HepG2 cells treated with ab70064 at 1/500 a dilution, with and without the immunising peptide.
Anti-hnRNP G antibody (ab70064)参考文献
This product has been referenced in:
Shao R et al. Coordinate regulation of heterogeneous nuclear ribonucleoprotein dynamics by steroid hormones in the human fallopian tube and endometrium in vivo and in vitro. Am J Physiol Endocrinol Metab302:E1269-82 (2012).
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