概述

  • 产品名称Anti-hnRNP G抗体
    参阅全部 hnRNP G 一抗
  • 描述
    兔多克隆抗体to hnRNP G
  • 经测试应用适用于: IHC-P, WB, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide derived from the N terminus of human hnRNP G.

  • 阳性对照
    • HepG2 cells, HeLa cell extract.

性能

应用

Our Abpromise guarantee covers the use of ab70064 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration. PubMed: 22436695
WB 1/500 - 1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
ELISA 1/5000.
ICC/IF 1/500 - 1/1000.

靶标

  • 相关性Heterogeneous nuclear ribonuclearproteins (hnRNPs) are nucleic acid binding proteins and have critical roles in DNA repair, telomere regulation, and transcriptional gene regulation. hnRNP G has tumor suppressive activity and this RNA binding protein which may be involved in pre-mRNA splicing.
  • 细胞定位Nuclear
  • 数据库链接
  • 别名
    • Glycoprotein p43 antibody
    • Heterogeneous nuclear ribonucleoprotein G antibody
    • hnRNP G antibody
    • HNRNPG antibody
    • HNRPG antibody
    • RBMX antibody
    • RBMXP1 antibody
    • RBMXRT antibody
    • RNA binding motif protein, X chromosome antibody
    • RNA binding motif protein, X linked antibody
    • RNMX antibody
    see all

Anti-hnRNP G antibody 图像

  • All lanes : Anti-hnRNP G antibody (ab70064) at 1/500 dilution

    Lane 1 : Extracts from HeLa cells
    Lane 2 : Extracts from HeLa cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 42 kDa
    Observed band size : 42 kDa
    Additional bands at : 80 kDa. We are unsure as to the identity of these extra bands.
  • HepG2 cells treated with ab70064 at 1/500 a dilution, with and without the immunising peptide.

Anti-hnRNP G antibody (ab70064)参考文献

This product has been referenced in:
  • Shao R  et al. Coordinate regulation of heterogeneous nuclear ribonucleoprotein dynamics by steroid hormones in the human fallopian tube and endometrium in vivo and in vitro. Am J Physiol Endocrinol Metab 302:E1269-82 (2012). WB, IHC-P ; Human . Read more (PubMed: 22436695) »

See 1 Publication for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (293T)
Loading amount 30000 cells
Specification 293T
Gel Running Conditions Reduced Denaturing (4-20% TrisGlycine)
Blocking step 5% Millk and 1% BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Feb 15 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"