概述

  • 产品名称Anti-hnRNP F抗体
    参阅全部 hnRNP F 一抗
  • 描述
    兔多克隆抗体to hnRNP F
  • 经测试应用适用于: ICC/IF, WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Cow
  • 免疫原

    A region within synthetic peptide: LNSTTGASNG AYSSQVMQGM GVSAAQATYS GLESQSVSGC YGAGYSGQNS, corresponding to C terminal amino acids 361-410 of Human hnRNP F.

  • 阳性对照
    • WB: HepG2 cell lysate. IHC-P: Human kidney tissue.

性能

应用

Our Abpromise guarantee covers the use of ab50982 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 46 kDa).Can be blocked with hnRNP F peptide (ab197136). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/62500.

IHC-P Use a concentration of 4 - 8 µg/ml.

靶标

  • 功能Component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Plays a role in the regulation of alternative splicing events. Binds G-rich sequences in pre-mRNAs and keeps target RNA in an unfolded state.
  • 组织特异性Expressed ubiquitously.
  • 序列相似性Contains 3 RRM (RNA recognition motif) domains.
  • 结构域The N-terminal RRM domains are responsible for recognizing the G-tract of BCL-X RNA.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
    Sumoylated.
  • 细胞定位Nucleus > nucleoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Heterogeneous nuclear ribonucleoprotein F antibody
    • hnRNP F antibody
    • HnRNP F protein antibody
    • Hnrnpf antibody
    • HNRPF antibody
    • HNRPF_HUMAN antibody
    • mcs94 1 antibody
    • MGC110997 antibody
    • N-terminally processed antibody
    • Nucleolin like protein antibody
    • Nucleolin like protein mcs94 1 antibody
    • Nucleolin-like protein mcs94-1 antibody
    • OK/SW-cl.23 antibody
    • OTTHUMP00000019482 antibody
    • OTTHUMP00000043413 antibody
    • OTTHUMP00000043414 antibody
    • Ribonucleoprotein F antibody
    see all

Anti-hnRNP F antibody 图像

  • All lanes : Anti-hnRNP F antibody (ab50982) at 1.25 µg/ml

    Lane 1 : MW marker
    Lane 2 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution


    Predicted band size : 46 kDa
    Observed band size : 48 kDa (why is the actual band size different from the predicted?)
  • ab50982, at a concentration of 4 µg/ml, staining hnRNP F in paraffin embedded human kidney tissue sections by Immunohistochemistry. Cells with positive label are epithelial cells of renal tubule (indicated by arrows). Magnification: 400x.
  • ICC/IF image of ab50982 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab50982, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab50982 staining hnRNP F in primary mouse oligodendrocytes (image shows 4 days in vitro) by Immunocytochemistry/ Immunofluorescence.
    hnRNP F is present in the nucleus and the cytoplasm of oligodendrocytes. In addition to its prominent nuclear localization (yellow arrowheads), hnRNP F is present in granular structures throughout the cytoplasm (white arrows). Insets show enlarged areas.

Anti-hnRNP F antibody (ab50982)参考文献

This product has been referenced in:
  • Vickers TA & Crooke ST Antisense oligonucleotides capable of promoting specific target mRNA reduction via competing RNase H1-dependent and independent mechanisms. PLoS One 9:e108625 (2014). WB . Read more (PubMed: 25299183) »
  • White R  et al. Heterogeneous Nuclear Ribonucleoprotein (hnRNP) F Is a Novel Component of Oligodendroglial RNA Transport Granules Contributing to Regulation of Myelin Basic Protein (MBP) Synthesis. J Biol Chem 287:1742-54 (2012). WB, ICC/IF ; Mouse . Read more (PubMed: 22128153) »

See all 2 Publications for this product

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (10)
Sample Rat Tissue lysate - whole (brain)
Specification brain
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
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提交于 Jun 20 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"