Anti-hnRNP F抗体[5F5] (ab118028)

概述

  • 产品名称Anti-hnRNP F抗体[5F5]
    参阅全部 hnRNP F 一抗
  • 描述
    小鼠单克隆抗体[5F5] to hnRNP F
  • 经测试应用适用于: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length Human hnRNP F produced in HEK293T cells (NP_004957).

  • 阳性对照
    • HEK293T cell lysate transfected with pCMV6-ENTRY hnRNP F cDNA; Human Kidney, liver, ovary adenocarcinoma, prostate, and bladder carcinoma tissues; COS7 cells transiently transfected by pCMV6-ENTRY hnRNP F; HepG2, HeLa and Jurkat cells.
  • 常规说明Dilute in PBS (pH7.3) before use. Stable for 12 months from date of receipt.

性能

应用

Our Abpromise guarantee covers the use of ab118028 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000. Predicted molecular weight: 46 kDa.
IHC-P 1/50.
Flow Cyt 1/100. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC/IF 1/50 - 1/100.

靶标

  • 功能Component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Plays a role in the regulation of alternative splicing events. Binds G-rich sequences in pre-mRNAs and keeps target RNA in an unfolded state.
  • 组织特异性Expressed ubiquitously.
  • 序列相似性Contains 3 RRM (RNA recognition motif) domains.
  • 结构域The N-terminal RRM domains are responsible for recognizing the G-tract of BCL-X RNA.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
    Sumoylated.
  • 细胞定位Nucleus > nucleoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Heterogeneous nuclear ribonucleoprotein F antibody
    • hnRNP F antibody
    • HnRNP F protein antibody
    • Hnrnpf antibody
    • HNRPF antibody
    • HNRPF_HUMAN antibody
    • mcs94 1 antibody
    • MGC110997 antibody
    • N-terminally processed antibody
    • Nucleolin like protein antibody
    • Nucleolin like protein mcs94 1 antibody
    • Nucleolin-like protein mcs94-1 antibody
    • OK/SW-cl.23 antibody
    • OTTHUMP00000019482 antibody
    • OTTHUMP00000043413 antibody
    • OTTHUMP00000043414 antibody
    • Ribonucleoprotein F antibody
    see all

Anti-hnRNP F antibody [5F5] 图像

  • All lanes : Anti-hnRNP F antibody [5F5] (ab118028) at 1/2000 dilution

    Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control
    Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY hnRNP F cDNA

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 46 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC201465)
  • ab118028, at 1/50 dilution, staining hnRNP F in paraffin-embedded Human kidney tissue by Immunohistochemistry.
  • ab118028, at 1/50 dilution, staining hnRNP F in paraffin-embedded Human liver tissue by Immunohistochemistry.
  • ab118028, at 1/50 dilution, staining hnRNP F in paraffin-embedded Human ovary adenocarcinoma tissue by Immunohistochemistry.
  • ab118028, at 1/50 dilution, staining hnRNP F in paraffin-embedded Human prostate tissue by Immunohistochemistry.
  • ab118028, at 1/50 dilution, staining hnRNP F in paraffin-embedded Human bladder carcinoma tissue by Immunohistochemistry.
  • ab118028, at 1/50 dilution, staining hnRNP F in COS7 cells transiently transfected by pCMV6-ENTRY hnRNP F by Immunofluorescence.
  • ab118028, at 1/50 dilution, staining hnRNP F in HepG2 cells by Immunofluorescence.
  • Flow cytometric Analysis of HeLa cells, using ab118028 at 1/100 (Red), compared to a nonspecific negative control antibody,(Blue).
  • Flow cytometric Analysis of Jurkat cells, using ab118028 at 1/100 (Red), compared to a nonspecific negative control antibody,(Blue).

Anti-hnRNP F antibody [5F5] (ab118028)参考文献

ab118028 has not yet been referenced specifically in any publications.

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