概述

  • 产品名称
    Anti-hnRNP D/AUF1抗体
    参阅全部 hnRNP D/AUF1 一抗
  • 描述
    兔多克隆抗体to hnRNP D/AUF1
  • 经测试应用
    适用于: ICC/IF, WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Dog, Zebrafish
  • 免疫原

    A region within synthetic peptide: AAAAAGSGAG TGGGTASGGT EGGSAESEGA KIDASKNEED EGKMFIGGLS, corresponding to N terminal amino acids 37-86 of Human hnRNP D/AUF1

  • 阳性对照
    • Jurkat cell lysate. Intestine.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度
    Protein A purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab50692 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 4 - 8 µg/ml.

靶标

  • 功能
    Binds with high affinity to RNA molecules that contain AU-rich elements (AREs) found within the 3'-UTR of many proto-oncogenes and cytokine mRNAs. Also binds to double- and single-stranded DNA sequences in a specific manner and functions a transcription factor. Each of the RNA-binding domains specifically can bind solely to a single-stranded non-monotonous 5'-UUAG-3' sequence and also weaker to the single-stranded 5'-TTAGGG-3' telomeric DNA repeat. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats. Binding of RRM1 to DNA inhibits the formation of DNA quadruplex structure which may play a role in telomere elongation. May be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain.
  • 序列相似性
    Contains 2 RRM (RNA recognition motif) domains.
  • 翻译后修饰
    Arg-345 is dimethylated, probably to asymmetric dimethylarginine.
    Methylated by PRMT1, in an insulin-dependent manner. The PRMT1-mediated methylation regulates tyrosine phosphorylation.
  • 细胞定位
    Nucleus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Component of ribonucleosomes.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ARE binding protein AUFI type A antibody
    • AU-rich element RNA-binding protein 1 antibody
    • AUF antibody
    • AUF1 antibody
    • AUF1A antibody
    • Heterogeneous nuclear ribonucleoprotein D0 antibody
    • hnRNP D antibody
    • hnRNP D0 antibody
    • Hnrnpd antibody
    • hnRNPD0 antibody
    • HNRPD antibody
    • HNRPD_HUMAN antibody
    • P37 antibody
    see all

Anti-hnRNP D/AUF1 antibody 图像

  • All lanes : Anti-hnRNP D/AUF1 antibody (ab50692) at 1.25 µg/ml

    Lane 1 : MW ladder
    Lane 2 : Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 38 kDa
    Observed band size : 38 kDa
    Additional bands at : 40 kDa. We are unsure as to the identity of these extra bands.
  • Staining of hnRNP D/AUF1 on human intestine tissue sections, using ab50692 at 8ug/ml. Expression is detected on the epithelial cells of the intestinal villus (arrows).
  • ab50692 stained HCT116 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab50692 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-hnRNP D/AUF1 antibody (ab50692)参考文献

This product has been referenced in:
  • Kumar M  et al. Nuclear heterogeneous nuclear ribonucleoprotein D is associated with poor prognosis and interactome analysis reveals its novel binding partners in oral cancer. J Transl Med 13:285 (2015). IHC-P ; Human . Read more (PubMed: 26318153) »
  • Hendrayani SF  et al. The cytokine IL-6 reactivates breast stromal fibroblasts through transcription factor STAT3-dependent up-regulation of the RNA-binding protein AUF1. J Biol Chem 289:30962-76 (2014). Read more (PubMed: 25231991) »

See all 5 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (head and neck cancer)
Specification
head and neck cancer
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris EDTA pH=9.0
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 27°C
Username

Dr. Satyendra Tripathi

Verified customer

提交于 Jul 19 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册