Anti-hnRNP C1 + C2 (phospho S260)抗体(ab61796)

概述

  • 产品名称Anti-hnRNP C1 + C2 (phospho S260)抗体
    参阅全部 hnRNP C1 + C2 一抗
  • 描述
    兔多克隆抗体to hnRNP C1 + C2 (phospho S260)
  • 特异性ab61796 detects endogenous levels of hnRNP C1 + C2 only when phosphorylated at serine 260.
  • 经测试应用适用于: ELISA, IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic phosphopeptide derived from human hnRNP C1 + C2 around the phosphorylation site of serine 260 (D-D-SP-A-E).

  • 阳性对照
    • Human brain tissue and 293 cell extracts treated with H2O2 (100uM, 15mins).

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab61796 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab61796 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA
IHC-P
WB
  • 应用说明ELISA: 1/20000.
    IHC-P: 1/50 - 1/100.
    WB: 1/500 - 1/1000. Detects bands of approximately 40, 45 kDa (predicted molecular weight: 34 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Binds pre-mRNA and nucleates the assembly of 40S hnRNP particles (PubMed:8264621). Specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs, a modification present at internal sites of mRNAs that affects mRNA splicing, processing and stability. M6A alters the local structure in mRNAs and long non-coding RNAs (lncRNAs) via a mechanism named 'm(6)A-switch' to facilitate binding of HNRNPC, leading to regulation of mRNA splicing (PubMed:25719671). Single HNRNPC tetramers bind 230-240 nucleotides. Trimers of HNRNPC tetramers bind 700 nucleotides. May play a role in the early steps of spliceosome assembly and pre-mRNA splicing. Interacts with poly-U tracts in the 3'-UTR or 5'-UTR of mRNA and modulates the stability and the level of translation of bound mRNA molecules (PubMed:12509468, PubMed:16010978, PubMed:7567451, PubMed:8264621).
    • 序列相似性Belongs to the RRM HNRPC family. RALY subfamily.
      Contains 1 RRM (RNA recognition motif) domain.
    • 翻译后修饰Phosphorylated on Ser-260 and Ser-299 in resting cells. Phosphorylated on Ser-253 and on 1 serine residue in the poly-Ser stretch at position 238 in response to hydrogen peroxide.
      Sumoylated. Sumoylation reduces affinity for mRNA.
    • 细胞定位Nucleus. Component of ribonucleosomes.
    • Information by UniProt
    • 数据库链接
    • 别名
      • C1 antibody
      • C2 antibody
      • Heterogeneous nuclear ribonucleoprotein C (C1/C2) antibody
      • Heterogeneous nuclear ribonucleoprotein C antibody
      • Heterogeneous nuclear ribonucleoproteins C1/C2 antibody
      • HNRNP antibody
      • hnRNP C1 / hnRNP C2 antibody
      • hnRNP C1/C2 antibody
      • Hnrnpc antibody
      • HNRPC antibody
      • HNRPC_HUMAN antibody
      • MGC104306 antibody
      • MGC105117 antibody
      • MGC117353 antibody
      • MGC131677 antibody
      • Nuclear ribonucleoprotein particle C1 protein antibody
      • Nuclear ribonucleoprotein particle C2 protein antibody
      • SNRPC antibody
      see all

    Anti-hnRNP C1 + C2 (phospho S260) antibody 图像

    • All lanes : Anti-hnRNP C1 + C2 (phospho S260) antibody (ab61796) at 1/500 dilution

      Lane 1 : 293 cell extracts treated with H2O2 (100uM, 15mins)
      Lane 2 : 293 cell extracts treated with H2O2 (100uM, 15mins) with immunising peptide at 5 µg

      Lysates/proteins at 5 µg per lane.


      Predicted band size : 34 kDa
      Observed band size : 45 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 40 kDa. We are unsure as to the identity of these extra bands.
    • ab61796 at 1/50 - 1/100 dilution staining hnRNP C1 + C2 in human brain Left apical lobe by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide.

    Anti-hnRNP C1 + C2 (phospho S260) antibody (ab61796)参考文献

    This product has been referenced in:
    • Chen S  et al. Reduced expression of lamin A/C results in modified cell signaling and metabolism coupled with changes in expression of structural proteins. J Proteome Res 8:5196-211 (2009). Read more (PubMed: 19775189) »

    See 1 Publication for this product

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