概述

  • 产品名称Anti-hnRNP C1 + C2抗体[EP3034Y]
    参阅全部 hnRNP C1 + C2 一抗
  • 描述
    兔单克隆抗体[EP3034Y] to hnRNP C1 + C2
  • 经测试应用适用于: WB, IHC-P, ICCmore details
    不适用于: Flow Cyt or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues near the N-terminus of human hnRNP C1 + C2

  • 阳性对照
    • HeLa, 293T, MCF7 and human cerebellum lysates; human breast carcinoma tissue.
  • 常规说明

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

应用

Our Abpromise guarantee covers the use of ab75822 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/50000 - 1/100000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/100 - 1/250.
  • 应用说明Is unsuitable for Flow Cyt or IP.
  • 靶标

    • 功能Binds pre-mRNA and nucleates the assembly of 40S hnRNP particles (PubMed:8264621). Specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs, a modification present at internal sites of mRNAs that affects mRNA splicing, processing and stability. M6A alters the local structure in mRNAs and long non-coding RNAs (lncRNAs) via a mechanism named 'm(6)A-switch' to facilitate binding of HNRNPC, leading to regulation of mRNA splicing (PubMed:25719671). Single HNRNPC tetramers bind 230-240 nucleotides. Trimers of HNRNPC tetramers bind 700 nucleotides. May play a role in the early steps of spliceosome assembly and pre-mRNA splicing. Interacts with poly-U tracts in the 3'-UTR or 5'-UTR of mRNA and modulates the stability and the level of translation of bound mRNA molecules (PubMed:12509468, PubMed:16010978, PubMed:7567451, PubMed:8264621).
    • 序列相似性Belongs to the RRM HNRPC family. RALY subfamily.
      Contains 1 RRM (RNA recognition motif) domain.
    • 翻译后修饰Phosphorylated on Ser-260 and Ser-299 in resting cells. Phosphorylated on Ser-253 and on 1 serine residue in the poly-Ser stretch at position 238 in response to hydrogen peroxide.
      Sumoylated. Sumoylation reduces affinity for mRNA.
    • 细胞定位Nucleus. Component of ribonucleosomes.
    • Information by UniProt
    • 数据库链接
    • 别名
      • C1 antibody
      • C2 antibody
      • Heterogeneous nuclear ribonucleoprotein C (C1/C2) antibody
      • Heterogeneous nuclear ribonucleoprotein C antibody
      • Heterogeneous nuclear ribonucleoproteins C1/C2 antibody
      • HNRNP antibody
      • hnRNP C1 / hnRNP C2 antibody
      • hnRNP C1/C2 antibody
      • Hnrnpc antibody
      • HNRPC antibody
      • HNRPC_HUMAN antibody
      • MGC104306 antibody
      • MGC105117 antibody
      • MGC117353 antibody
      • MGC131677 antibody
      • Nuclear ribonucleoprotein particle C1 protein antibody
      • Nuclear ribonucleoprotein particle C2 protein antibody
      • SNRPC antibody
      see all

    Anti-hnRNP C1 + C2 antibody [EP3034Y] 图像

    • ab75822 at 1/100 dilution staining hnRNP C1 + C2 in human breast carcinoma by Immunohistochemistry, Paraffin-embedded tissue.
    • All lanes : Anti-hnRNP C1 + C2 antibody [EP3034Y] (ab75822) at 1/100000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : MCF7 cell lysate
      Lane 4 : human cerebellum lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 34 kDa
      Observed band size : 34 kDa

    Anti-hnRNP C1 + C2 antibody [EP3034Y] (ab75822)参考文献

    This product has been referenced in:
    • Akhrymuk I  et al. Evasion of the innate immune response: the Old World alphavirus nsP2 protein induces rapid degradation of Rpb1, a catalytic subunit of RNA polymerase II. J Virol 86:7180-91 (2012). WB . Read more (PubMed: 22514352) »

    See 1 Publication for this product

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