概述

  • 产品名称Anti-hnRNP A2B1抗体
    参阅全部 hnRNP A2B1 一抗
  • 描述
    兔多克隆抗体to hnRNP A2B1
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Chicken, Dog, Xenopus laevis
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human hnRNP A2B1.

    (Peptide available as ab31643.)

  • 阳性对照
    • This antibody gave a positive signal in MCF7 cells and Human breast adenocarcinoma tissue section.

性能

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应用

Our Abpromise guarantee covers the use of ab24409 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 35, 38 kDa (predicted molecular weight: 35 , 37 kDa).
ICC/IF Use a concentration of 1 µg/ml.

靶标

  • 功能Involved with pre-mRNA processing. Forms complexes (ribonucleosomes) with at least 20 other different hnRNP and heterogeneous nuclear RNA in the nucleous.
  • 序列相似性Contains 2 RRM (RNA recognition motif) domains.
  • 细胞定位Nucleus > nucleoplasm. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Component of ribonucleosomes. Predominantly nucleoplasmic, however isoform A2 is also found in the cytoplasm of cells in some tissues. Not found in the nucleolus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Heterogeneous nuclear ribonucleoprotein A2 antibody
    • Heterogeneous nuclear ribonucleoprotein A2/B1 antibody
    • Heterogeneous nuclear ribonucleoprotein B1 antibody
    • Heterogeneous nuclear ribonucleoproteins A2/B1 antibody
    • hnRNP A2 / hnRNP B1 antibody
    • hnRNP A2 antibody
    • hnRNP A2/B1 antibody
    • hnRNP B1 antibody
    • hnRNP-A2 antibody
    • hnRNP-B1 antibody
    • hnRNPA2 antibody
    • Hnrnpa2b1 antibody
    • hnRNPB1 antibody
    • HNRPA2 antibody
    • HNRPA2B1 antibody
    • HNRPB1 antibody
    • Nuclear ribonucleoprotein particle A2 protein antibody
    • RNP A2 antibody
    • RNP B1 antibody
    • RNP-A2 antibody
    • RNP-B1 antibody
    • RNPA2 antibody
    • RNPB1 antibody
    • ROA2_HUMAN antibody
    • SNRPB1 antibody
    see all

Anti-hnRNP A2B1 antibody 图像

  • Anti-hnRNP A2B1 antibody (ab24409) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg

    Secondary
    Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 35 , 37 kDa
    Observed band size : 35,38 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 30 kDa (possible cross reactivity),45 kDa (possible cross reactivity),80 kDa (possible cross reactivity).
  • ICC/IF image of ab24409 stained human MCF7 cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab24409, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HeLa, HEK 293 and HepG2 cells.

  • IHC image of ab24409 staining in Human breast adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab24409, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-hnRNP A2B1 antibody (ab24409)参考文献

ab24409 has not yet been referenced specifically in any publications.

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