Anti-HNF1 beta抗体(ab59118)

概述

  • 产品名称Anti-HNF1 beta抗体
    参阅全部 HNF1 beta 一抗
  • 描述
    山羊多克隆抗体to HNF1 beta
  • 特异性This antibody is expected to recognize both reported isoforms
  • 经测试应用适用于: WB, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Dog, Pig, Xenopus laevis, Zebrafish
  • 免疫原

    Synthetic peptide:

    QAYDRQKNPSKEER

    by a Cysteine residue linker, corresponding to amino acids 248-261 of Human TCF2

  • 阳性对照
    • Human Liver lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab59118 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).
ELISA 1/64000.
ICC/IF Use at an assay dependent concentration.

靶标

  • 功能Transcription factor, probably binds to the inverted palindrome 5'-GTTAATNATTAAC-3'.
  • 疾病相关Defects in HNF1B are the cause of renal cysts and diabetes syndrome (RCAD) [MIM:137920]; also called maturity-onset diabetes of the young type 5 (MODY5) or familial hypoplastic glomerulocystic kidney disease (GCKD). RCAD is an autosomal dominant disorder comprising non-diabetic renal disease resulting from abnormal renal development, and diabetes, which in some cases occurs earlier than age 25 years and is thus consistent with a diagnosis of maturity-onset diabetes of the young (MODY5). The renal disease is highly variable and includes renal cysts, glomerular tufts, aberrant nephrogenesis, primitive tubules, irregular collecting systems, oligomeganephronia, enlarged renal pelves, abnormal calyces, small kidney, single kidney, horseshoe kidney, and hyperuricemic nephropathy.
    Defects in HNF1B may be rare genetic risk factor contributing to the development of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853]. NIDDM is characterized by an autosomal dominant mode of inheritance, onset during adulthood and insulin resistance.
    Defects in HNF1B may be a cause of susceptibility to prostate cancer hereditary type 11 (HPC11) [MIM:611955]. It is a condition associated with familial predisposition to cancer of the prostate. Most prostate cancers are adenocarcinomas that develop in the acini of the prostatic ducts. Other rare histopathologic types of prostate cancer that occur in approximately 5% of patients include small cell carcinoma, mucinous carcinoma, prostatic ductal carcinoma, transitional cell carcinoma, squamous cell carcinoma, basal cell carcinoma, adenoid cystic carcinoma (basaloid), signet-ring cell carcinoma and neuroendocrine carcinoma.
  • 序列相似性Belongs to the HNF1 homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FJHN antibody
    • Hepatocyte nuclear factor 1 beta antibody
    • Hepatocyte nuclear factor 1-beta antibody
    • HNF 1B antibody
    • HNF 2 antibody
    • HNF-1-beta antibody
    • HNF-1B antibody
    • HNF1 beta antibody
    • HNF1 homeobox B antibody
    • HNF1B antibody
    • HNF1B_HUMAN antibody
    • HNF1beta antibody
    • HNF2 antibody
    • Homeoprotein LF B3 antibody
    • Homeoprotein LFB3 antibody
    • HPC11 antibody
    • LF B3 antibody
    • LFB3 antibody
    • MODY 5 antibody
    • MODY5 antibody
    • TCF 2 antibody
    • TCF 2 protein antibody
    • TCF-2 antibody
    • TCF2 antibody
    • TCF2 protein antibody
    • Transcription factor 2 antibody
    • Transcription factor 2 hepatic antibody
    • Variant hepatic nuclear factor 1 antibody
    • Variant hepatic nuclear factor antibody
    • VHNF 1 antibody
    • vHNF1 antibody
    see all

Anti-HNF1 beta antibody 图像

  • Anti-HNF1 beta antibody (ab59118) at 0.1 µg/ml + Human liver lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 61 kDa
    Observed band size : 61 kDa
    Additional bands at : 100 kDa. We are unsure as to the identity of these extra bands.
    The 100kDa band observed was successfully blocked by incubation with the immunizing peptide, however its identity is not known.
  • Definitive endoderm derived from culturing human embryonic stem cells (HUES4) with Inhibin beta a (ab50051) and Wnt3a (ab81484) was further differentiated to primitive gut tube as described in Kroon et al, Nat Biotechnol. (2008). KGF (ab9601) was added to the media to direct differentiation towards primitive gut tube. These images were taken 3 days after addition of KGF, where the primitive gut tube marker TCF2 (ab59118 in green) is present. Dapi staining is shown in blue. Magnification of top panels: 100x, bottom panels: 200x. Negative controls of fluorescently conjugated secondary antibodies only, showed no staining. For more information on this immunofluorescence, please see abreview.

    See Abreview

Anti-HNF1 beta antibody (ab59118)参考文献

ab59118 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us.

We are happy to help customers find the most suitable products for their research purposes; however we are not specialized in this particular field and do not want to recommend a product that may be unsuitable....

Read More

Thank you for sending your customer's protocol and data. The protocol is similar to the protocol used in a positive review on our datasheet from February 2011, using different cells (human embryonic stem cells). I do not have suggestions to improve ...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (primitive gut tube from human embryonic stem cells)
Specification primitive gut tube from human embryonic stem cells
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% triton
Blocking step Dako Protein Block (0.25% caesin) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.25% · Temperature: 25°C
Username

Ms. Emily Moorefield

Verified customer

提交于 Feb 24 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"