Anti-HNF-4-alpha + HNF-4-gamma抗体
兔多克隆抗体to HNF-4-alpha + HNF-4-gamma
Rabbit polyclonal to HNF-4-alpha + HNF-4-gamma antibody detects endogenous levels of total HNF-4-alpha/gamma protein.
Synthetic peptide corresponding to Human HNF-4-alpha + HNF-4-gamma (internal sequence).
Extracts from HeLa cells, HepG2 cells and 293 cells.
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg 2+ and Ca 2+), 150mM Sodium chloride, pH 7.4
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Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml.
1/500 - 1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
Anti-HNF-4-alpha + HNF-4-gamma antibody 图像
Western blot - Anti-HNF-4-alpha + HNF-4-gamma antibody (ab64911)
All lanes : Anti-HNF-4-alpha + HNF-4-gamma antibody (ab64911) at 1/500 dilution Lane 1 : extracts from HeLa cells Lane 2 : extracts from HepG2 cells Lane 3 : extracts from 293 cells Lane 4 : extracts from 293 cells with immunising peptide at 10 µg Lysates/proteins at 30 µg per lane. Predicted band size : 52 kDa Observed band size : 52 kDa Additional bands at : 30 kDa,40 kDa,65 kDa,75 kDa,90 kDa. We are unsure as to the identity of these extra bands.
Immunocytochemistry/ Immunofluorescence - Anti-HNF-4-alpha + HNF-4-gamma antibody (ab64911)
ICC/IF image of ab64911 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64911, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Anti-HNF-4-alpha + HNF-4-gamma antibody (ab64911)参考文献
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