Application
Western blot
Loading amount
15 µg
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (4-12% Bis-Tris Gel, MES running buffer)
Sample
Human Cell lysate - whole cell (Human Embryonic Kidney)
Specification
Human Embryonic Kidney
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Blocking Agent
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: anti-Mouse IgG, HRP-conjugate
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL+
Bands
Specific: 35 kDa Non-specific: 17 kDa
Exposure
5 second(s)
Negative control
No bands detected.
Positive control
Showed 35 kDa band, very faint band at 17 kDa.
Additional data
Additional Notes
Used PVDF membrane.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Mar 18 2014