The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用说明ICC/IF: Use at an assay dependent dilution.
IHC-P: 1/100 - 1/200 for 20 - 30 minutes at RT. For staining of formalin-fixed tissues, digest sections with trypsin at 1mg/ml PBS, 10 min at 37°C or Protease XXV at 1mg/ml PBS for 5 minutes at 37°C.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
相关性Cytokeratins are intermediate filament keratins found in the intracytoplasmic cytoskeleton of epithelial tissue. There are two types of Cytokeratins: the low weight, acidic type I cytokeratins and the high weight, basic or neutral type II. Cytokeratins are usually found in pairs comprising a type I Cytokeratin and a type II cytokeratin. The high molecular weight cytokeratins, which are the basic or neutral cytokeratins, comprise subtypes CK1 (67), CK2 (65.5), CK3 (64), CK4 (59), CK5 (58), CK6 (56), CK7 (54), CK8 (52.5) and CK9. The low molecular weight cytokeratins, which are the acidic cytokeratins, comprise subtypes CK10 (56.5), CK12 (56), CK13 (53), CK14 (50), CK16( 48), CK17 (46), CK18 (45), CK19(48) and CK20(46).
Formalin-fixed, paraffin-embedded human skin stained with ab80823 at 1/100 dilution using a peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of epithelial cells.
Anti-HMW Cytokeratin antibody [AE3] (ab80823)参考文献
This product has been referenced in:
Eichner R et al. Classification of epidermal keratins according to their immunoreactivity, isoelectric point, and mode of expression. J Cell Biol98:1388-96 (1984).
Read more (PubMed: 6201491) »
Cooper D et al. The use of aIF, AE1, and AE3 monoclonal antibodies for the identification and classification of mammalian epithelial keratins. Differentiation28:30-5 (1984).
Read more (PubMed: 6083891) »