Anti-HMW Cytokeratin抗体[34betaE12] (ab86725)


  • 产品名称Anti-HMW Cytokeratin抗体[34betaE12]
    参阅全部 HMW Cytokeratin 一抗
  • 描述
    小鼠单克隆抗体[34betaE12] to HMW Cytokeratin
  • 特异性ab86725 recognizes cytokeratin 1, 5, 10 and 14
  • 经测试应用适用于: IHC-P, ICC/IF, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Rat, Dog, Human
  • 免疫原

    HMW CK 34betaE12

  • 阳性对照
    • Human, mouse or rat skin, prostate, squamous cell carcinoma or basal cell carcinoma tissue. This antibody gave a positive result when used in the following methanol fixed cell lines: DU145.



Our Abpromise guarantee covers the use of ab86725 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50 - 1/100.
ICC/IF Use a concentration of 10 µg/ml.
Flow Cyt 1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.


Anti-HMW Cytokeratin antibody [34betaE12] 图像

  • ab86725, at 1/50 dilution, staining Human HMW Cytokeratin in prostate, using Immunohistochemistry, Formalin/PFA-fixed paraffin embedded tissue.
  • Overlay histogram showing A431 cells stained with ab86725 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab86725, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • ICC/IF image of ab86725 stained DU145 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab86725 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-HMW Cytokeratin antibody [34betaE12] (ab86725)参考文献

This product has been referenced in:
  • Kuramoto G  et al. Preventive effect of oral mucosal epithelial cell sheets on intrauterine adhesions. Hum Reprod 30:406-16 (2015). Read more (PubMed: 25475585) »
  • Moinfar F  et al. Use of keratin 35betaE12 as an adjunct in the diagnosis of mammary intraepithelial neoplasia-ductal type--benign and malignant intraductal proliferations. Am J Surg Pathol 23:1048-58 (1999). Read more (PubMed: 10478664) »

See all 5 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Pig Tissue sections (Ureter)
Specification Ureter
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM Citric Acid pH6 or EDTA 1mM pH8
Permeabilization No
Blocking step Serum as blocking agent for 15 minute(s) · Concentration: 10% · Temperature: 21°C

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提交于 Aug 10 2011