概述

  • 产品名称Anti-HMGN3抗体
    参阅全部 HMGN3 一抗
  • 描述
    兔多克隆抗体to HMGN3
  • 经测试应用适用于: WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Horse, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat
  • 免疫原

    Synthetic peptide from a region between residue 60 and 99 of human HMGN3, NP_004233.1.

  • 阳性对照
    • Whole cell lysate from Hela cells.

性能

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应用

Our Abpromise guarantee covers the use of ab72233 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Detects a band of approximately 15, 16, 19, 51 kDa (predicted molecular weight: 11 kDa).
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Binds to nucleosomes, regulating chromatin structure and consequently, chromatin-dependent processes such as transcription, DNA replication and DNA repair. Affects both insulin and glucagon levels and modulates the expression of pancreatic genes involved in insulin secretion. Regulates the expression of the glucose transporter SLC2A2 by binding specifically to its promoter region and recruiting PDX1 and additional transcription factors. Regulates the expression of SLC6A9, a glycine transporter which regulates the glycine concentration in synaptic junctions in the central nervous system, by binding to its transcription start site. May play a role in ocular development and astrocyte function.
  • 组织特异性Expressed in kidney, lung, pancreas, testis, skeletal muscle, heart, thyroid gland, pituitary gland, prostate and uterus. Low expression in liver, spleen, placenta and ovaries.
  • 序列相似性Belongs to the HMGN family.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DKFZp686E20226 antibody
    • FLJ42187 antibody
    • High mobility group nucleosomal binding domain 3 antibody
    • High mobility group nucleosome-binding domain-containing protein 3 antibody
    • HMGN3 antibody
    • HMGN3_HUMAN antibody
    • OTTHUMP00000016772 antibody
    • OTTHUMP00000016773 antibody
    • PNAS 24 antibody
    • PNAS 25 antibody
    • PNAS24 antibody
    • PNAS25 antibody
    • Thyroid hormone receptor interacting protein 7 antibody
    • Thyroid receptor-interacting protein 7 antibody
    • TR-interacting protein 7 antibody
    • TRIP 7 antibody
    • TRIP-7 antibody
    • TRIP7 antibody
    see all

Anti-HMGN3 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling HMGN3 with ab72233 at 1/1000 (0.2µg/ml). Detection: DAB.
  • All lanes : Anti-HMGN3 antibody (ab72233) at 0.04 µg/ml

    Lane 1 : Whole cell lysate from Hela cells at 50 µg
    Lane 2 : Whole cell lysate from Hela cells at 15 µg
    Lane 3 : Whole cell lysate from Hela cells at 5 µg


    Predicted band size : 11 kDa
    Observed band size : 19 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 15 kDa,16 kDa,51 kDa. We are unsure as to the identity of these extra bands.
  • Immunoprecipitation/ Western Blot of HMGN3.
    Lane 1: ab72233 at 3µg/mg whole cell lysate.
    Lane 2: Control IgG.
    ab72233 at 1µg/ml for WB.
    Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
    Chemiluminescence with an exposure time of 3 seconds.

Anti-HMGN3 antibody (ab72233)参考文献

ab72233 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"