重组
RabMAb

Anti-HMGN2抗体[EPR7091] (ab124997)

概述

  • 产品名称
    Anti-HMGN2抗体[EPR7091]
    参阅全部 HMGN2 一抗
  • 描述
    兔单克隆抗体[EPR7091] to HMGN2
  • 经测试应用
    适用于: WB, IHC-P, ICCmore details
    不适用于: Flow Cyt or IP
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to residues in Human HMGN2.

  • 阳性对照
    • HL60, K562, HeLa, SH-SY5Y and Jurkat cell lysates; Human colon tissue.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
  • 纯度
    Tissue culture supernatant
  • 克隆
    单克隆
  • 克隆编号
    EPR7091
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab124997 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 9 kDa.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
ICC 1/100 - 1/250.
  • 应用说明
    Is unsuitable for Flow Cyt or IP.
  • 靶标

    • 功能
      Binds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer. May be involved in the process which maintains transcribable genes in an unique chromatin conformation.
    • 序列相似性
      Belongs to the HMGN family.
    • 翻译后修饰
      Phosphorylation favors cytoplasmic localization.
    • 细胞定位
      Nucleus. Cytoplasm. Cytoplasmic enrichment upon phosphorylation.
    • Information by UniProt
    • 数据库链接
    • 别名
      • High mobility group (nonhistone chromosomal) protein 17 antibody
      • high mobility group nucleosomal binding domain 2 antibody
      • High mobility group nucleosome-binding domain-containing protein 2 antibody
      • High mobility group protein N2 antibody
      • HMG17 antibody
      • HMGN2 antibody
      • HMGN2_HUMAN antibody
      • MGC5629 antibody
      • Non histone chromosomal protein HMG 17 antibody
      • Non-histone chromosomal protein HMG-17 antibody
      • Nonhistone chromosomal protein HMG 17 antibody
      • nonhistone chromosomal protein hmg-17 antibody
      see all

    图片

    • All lanes : Anti-HMGN2 antibody [EPR7091] (ab124997) at 1/1000 dilution

      Lane 1 : HL60 cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : SH-SY5Y cell lysate
      Lane 5 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size : 9 kDa
    • ab124997 at 1/250 dilution staining HMGN2 in paraffin-embedded Human colon tissue by Immunohistochemistry.

    文献

    ab124997 has not yet been referenced specifically in any publications.

    客户评价及客户问答

    There are currently no Customer reviews or Questions for ab124997.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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