Anti-HMGCS1抗体(ab155787)
Key features and details
- Rabbit polyclonal to HMGCS1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-HMGCS1抗体
参阅全部 HMGCS1 一抗 -
描述
兔多克隆抗体to HMGCS1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Cow -
免疫原
Recombinant fragment, corresponding to a region within amino acids 194-455 of Human HMGCS1 (Uniprot ID: Q01581).
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阳性对照
- 293T, A431, HeLa and Mouse liver whole cell lysates; Hela cells; Rat RT2 xenograft and Human CL15 xenograft tissues.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab155787于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 57 kDa.
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IHC-P |
1/100 - 1/1000.
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ICC/IF |
1/100 - 1/1000.
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说明 |
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WB
1/500 - 1/3000. Predicted molecular weight: 57 kDa. |
IHC-P
1/100 - 1/1000. |
ICC/IF
1/100 - 1/1000. |
靶标
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功能
This enzyme condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase. -
通路
Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 2/3. -
序列相似性
Belongs to the HMG-CoA synthase family. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 407767 Cow
- Entrez Gene: 3157 Human
- Entrez Gene: 100040592 Mouse
- Entrez Gene: 208715 Mouse
- Entrez Gene: 29637 Rat
- Omim: 142940 Human
- SwissProt: Q01581 Human
- SwissProt: Q8JZK9 Mouse
see all -
别名
- 3 hydroxy 3 methylglutaryl Coenzyme A synthase 1 (soluble) antibody
- 3-hydroxy-3-methylglutaryl coenzyme A synthase antibody
- cytoplasmic antibody
see all
图片
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All lanes : Anti-HMGCS1 antibody (ab155787) at 1/5000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 57 kDa
7.5% SDS PAGE -
Anti-HMGCS1 antibody (ab155787) at 1/1000 dilution + Mouse liver whole cell lysate at 30 µg
Predicted band size: 57 kDa
7.5% SDS PAGE -
Immunofluorescence analysis of methanol-fixed HeLa cells, labeling HMGCS1 with ab155787 at 1/500 dilution. Lower image shows cells co-stained with Hoechst 33342.
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Immunohistochemical analysis of paraffin-embedded Rat RT2 xenograft tissue, labeling HMGCS1 with ab155787 at 1/500 dilution.
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Immunohistochemical analysis of paraffin-embedded Human CL15 xenograft tissue, labeling HMGCS1 with ab155787 at 1/500 dilution.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab155787 被引用在 7 文献中.
- Tiong TY et al. Targeting the SREBP-1/Hsa-Mir-497/SCAP/FASN Oncometabolic Axis Inhibits the Cancer Stem-like and Chemoresistant Phenotype of Non-Small Cell Lung Carcinoma Cells. Int J Mol Sci 23:N/A (2022). PubMed: 35806291
- Zhou X et al. Qki regulates myelinogenesis through Srebp2-dependent cholesterol biosynthesis. Elife 10:N/A (2021). PubMed: 33942715
- Zhang J et al. Astrocytic YAP prevents the demyelination through promoting expression of cholesterol synthesis genes in experimental autoimmune encephalomyelitis. Cell Death Dis 12:907 (2021). PubMed: 34611127
- Lavado-García J et al. Multiplexed Quantitative Proteomic Analysis of HEK293 Provides Insights into Molecular Changes Associated with the Cell Density Effect, Transient Transfection, and Virus-Like Particle Production. J Proteome Res 19:1085-1099 (2020). PubMed: 31994890
- Kotula-Balak M et al. Towards understanding leydigioma: do G protein-coupled estrogen receptor and peroxisome proliferator-activated receptor regulate lipid metabolism and steroidogenesis in Leydig cell tumors? Protoplasma 257:1149-1163 (2020). PubMed: 32180008
- Kaymak I et al. Mevalonate Pathway Provides Ubiquinone to Maintain Pyrimidine Synthesis and Survival in p53-Deficient Cancer Cells Exposed to Metabolic Stress. Cancer Res 80:189-203 (2020). PubMed: 31744820
- Szántó M et al. Deletion of PARP-2 induces hepatic cholesterol accumulation and decrease in HDL levels. Biochim Biophys Acta 1842:594-602 (2014). Mouse . PubMed: 24365238