重组
RabMAb

Anti-HMGB2抗体[EPR6301] (ab124670)

概述

  • 产品名称
    Anti-HMGB2抗体[EPR6301]
    参阅全部 HMGB2 一抗
  • 描述
    兔单克隆抗体[EPR6301] to HMGB2
  • 经测试应用
    适用于: ICC/IF, WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human HMGB2 (N terminal). The exact sequence is proprietary.

  • 阳性对照
    • WB: K562, HeLa and PC12 cell lysates. ICC/IF: PC-12 cells. IHC-P: Human breast tissue.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Alternative versions available:

    Anti-HMGB2 antibody (Alexa Fluor® 647) [EPR6301] (ab207368)
    Anti-HMGB2 antibody (Alexa Fluor® 488) [EPR6301] (ab208388)

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 解离常数(KD
    KD = 6.03 x 10 -10 M
    Learn more about KD
  • 存储溶液
    PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • 纯度
    Tissue culture supernatant
  • 克隆
    单克隆
  • 克隆编号
    EPR6301
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab124670 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/500.
WB 1/10000 - 1/50000. Predicted molecular weight: 24 kDa.
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

靶标

图片

  • All lanes : Anti-HMGB2 antibody [EPR6301] (ab124670) at 1/10000 dilution

    Lane 1 : K562 cell lysates
    Lane 2 : HeLa cell lysates
    Lane 3 : PC12 cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat-anti-rabbit HRP at 1/2000 dilution

    Predicted band size : 24 kDa
  • Immunocytochemistry/Immunofluorescence analysis of PC-12 cells labelling HMGB2 with ab124670 at 1/500. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
    Control: PBS only.
    Nuclear counter stain: DAPI.

  • ab124670, at a 1/250 dilution, staining HMGB2 in paraffin embedded Human breast tissue by Immunohistochemistry.
  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

文献

ab124670 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
ChIP
Sample
Human Cell lysate - nuclear (HUVEC)
Negative control
olfactory region 8 (OR8)
Specification
HUVEC
Detection step
Real-time PCR
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 60 minute(s) and 0 second(s)
Specification of the cross-linking agent: 20 min EGS + 40 min with PFA w
Positive control
5’ prime UTR of ZNF335 and HIST2H2AC genes
Username

Dr. Theodore Georgomanolis

Verified customer

提交于 May 25 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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