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RabMAb

Anti-HMGB2抗体[EPR6301] (ab124670)

概述

  • 产品名称
    Anti-HMGB2抗体[EPR6301]
    参阅全部 HMGB2 一抗
  • 描述
    兔单克隆抗体[EPR6301] to HMGB2
  • 宿主
    Rabbit
  • 经测试应用
    适用于: ICC/IF, WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide within Human HMGB2 (N terminal). The exact sequence is proprietary.

  • 阳性对照
    • WB: K562, HeLa and PC12 cell lysates. ICC/IF: PC-12 cells. IHC-P: Human breast tissue.
  • 常规说明

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 解离常数(KD
    KD = 6.03 x 10 -10 M
    Learn more about KD
  • 存储溶液
    PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • 纯度
    Tissue culture supernatant
  • 克隆
    单克隆
  • 克隆编号
    EPR6301
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab124670 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/500.
WB 1/10000 - 1/50000. Predicted molecular weight: 24 kDa.
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

靶标

图片

  • All lanes : Anti-HMGB2 antibody [EPR6301] (ab124670) at 1/10000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : HMGB2 knockout HAP1 whole cell lysate
    Lane 3 : Hela whole cell lysate
    Lane 4 : K562 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 24 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab124670 observed at 24 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab124670 was shown to specifically react with HMGB2 in wild-type HAP1 cells as signal was lost in HMGB2 knockout cells. Wild-type and HMGB2 knockout samples were subjected to SDS-PAGE. Ab124670 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • All lanes : Anti-HMGB2 antibody [EPR6301] (ab124670) at 1/10000 dilution

    Lane 1 : K562 cell lysates
    Lane 2 : HeLa cell lysates
    Lane 3 : PC12 cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat-anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 24 kDa

  • Immunocytochemistry/Immunofluorescence analysis of PC-12 cells labelling HMGB2 with ab124670 at 1/500. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
    Control: PBS only.
    Nuclear counter stain: DAPI.

  • ab124670, at a 1/250 dilution, staining HMGB2 in paraffin embedded Human breast tissue by Immunohistochemistry.
  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

文献

This product has been referenced in:

See 1 Publication for this product

客户评价及客户问答

Application
ChIP
Sample
Human Cell lysate - nuclear (HUVEC)
Negative control
olfactory region 8 (OR8)
Specification
HUVEC
Detection step
Real-time PCR
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 60 minute(s) and 0 second(s)
Specification of the cross-linking agent: 20 min EGS + 40 min with PFA w
Positive control
5’ prime UTR of ZNF335 and HIST2H2AC genes
Username

Dr. Theodore Georgomanolis

Verified customer

提交于 May 25 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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