概述

  • 产品名称Anti-HMGA2抗体
    参阅全部 HMGA2 一抗
  • 描述
    兔多克隆抗体to HMGA2
  • 经测试应用适用于: IHC-P, IP, WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Recombinant fragment, corresponding to a region within N terminal amino acids 1 - 93 of Human HMGA2 (NP_003474).

  • 阳性对照
    • MOLT4 whole cell lysate, MCF7 cells, HepG2, HCT116, HeLa

性能

应用

Our Abpromise guarantee covers the use of ab97276 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/100 - 1/1000.
IP 1/100 - 1/500.
WB 1/500 - 1/3000. Predicted molecular weight: 12 kDa.
ICC/IF Use a concentration of 1 µg/ml.

靶标

  • 功能Functions as a transcriptional regulator. Functions in cell cycle regulation through CCNA2.
  • 疾病相关Note=A chromosomal aberration involving HMGA2 is associated with a subclass of benign mesenchymal tumors known as lipomas. Translocation t(3;12)(q27-q28;q13-q15) with LPP is shown in lipomas. HMGA2 is also fused with a number of other genes in lipomas.
    Note=A chromosomal aberration involving HMGA2 is associated with pulmonary chondroid hamartomas. Translocation t(3;12)(q27-q28;q14-q15) with LPP is detected in pulmonary chondroid hamartomas.
    Note=A chromosomal aberration involving HMGA2 is associated with parosteal lipomas. Translocation t(3;12)(q28;q14) with LPP is also shown in one parosteal lipoma.
    Note=A chromosomal aberration involving HMGA2 is found in uterine leiomyoma. Translocation t(12;14)(q15;q23-24) with RAD51L1. Chromosomal rearrangements involving HMGA2 do not seem to be the principle pathobiological mechanism in uterine leiomyoma.
  • 序列相似性Belongs to the HMGA family.
    Contains 3 A.T hook DNA-binding domains.
  • 发展阶段Expressed predominantly during embryogenesis.
  • 翻译后修饰Regulated by cell cycle-dependent phosphorylation which alters its DNA binding affinity.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • HMGA 2 antibody
    • BABL antibody
    • High mobility group (nonhistone chromosomal) protein isoform I C antibody
    • High mobility group (nonhistone chromosomal) protein isoform IC antibody
    • High Mobility Group AT hook 2 antibody
    • High Mobility Group AT hook protein 2 antibody
    • High mobility group AT-hook protein 2 antibody
    • High mobility group protein HMGI C antibody
    • High mobility group protein HMGI-C antibody
    • High mobility group protein HMGIC antibody
    • High mobility group protein I, isoform C antibody
    • HMGA2 antibody
    • HMGA2_HUMAN antibody
    • HMGI C antibody
    • HMGIC antibody
    • LIPO antibody
    • Non histone chromosomal architectural protein HMGI C antibody
    • Non histone chromosomal architectural protein HMGIC antibody
    • Pygmy antibody
    • STQTL9 antibody
    see all

Anti-HMGA2 antibody 图像

  • Anti-HMGA2 antibody (ab97276) at 1/1000 dilution + MOLT4 whole cell lysate at 30 µg

    Predicted band size : 12 kDa
  • ICC/IF image of ab97276 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab97276, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-HMGA2 antibody (ab97276) at 1/10000 dilution

    Lane 1 : HepG2
    Lane 2 : HCT116

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 12 kDa

Anti-HMGA2 antibody (ab97276)参考文献

This product has been referenced in:
  • Meijer HA  et al. Translational repression and eIF4A2 activity are critical for microRNA-mediated gene regulation. Science 340:82-5 (2013). WB . Read more (PubMed: 23559250) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Pig Cell lysate - whole cell (Primary Fetal Fibroblast)
Gel Running Conditions Reduced Denaturing (Mini-PROTEAN® TGX™ 4-20%)
Loading amount 50 µg
Specification Primary Fetal Fibroblast
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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提交于 Nov 21 2016

Application ChIP
Detection step Semiquantitative PCR
Sample Human Cell lysate - whole cell (HEK293)
Specification HEK293
Negative control DW No-antibody
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 8 minute(s) and 0 second(s)
Specification of the cross-linking agent: formaldehyde
Positive control input (whole lysate)
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Abcam user community

Verified customer

提交于 Sep 19 2014

The predicted size of HMGA2 is at 12 kDa, but it is also reported to migrate at higher size around 17-19 kDa probably due to PTM . (see publication: http://www.ncbi.nlm.nih.gov/pubmed/15026339)

The Uniprot database also shows HMGA2 has severa...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLA)
Loading amount 25 µg
Specification HeLA
Gel Running Conditions Reduced Denaturing (17.5)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Feb 01 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"