概述

  • 产品名称Anti-HMGA2抗体
    参阅全部 HMGA2 一抗
  • 描述
    兔多克隆抗体to HMGA2
  • 经测试应用适用于: WB, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to C-terminal of human HMGA2.

  • 阳性对照
    • SW579 cells. Breast carcinoma

性能

应用

Our Abpromise guarantee covers the use of ab52039 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. PubMed: 23184966
ICC/IF Use a concentration of 1 - 5 µg/ml.
IHC-P 1/50.

靶标

  • 功能Functions as a transcriptional regulator. Functions in cell cycle regulation through CCNA2.
  • 疾病相关Note=A chromosomal aberration involving HMGA2 is associated with a subclass of benign mesenchymal tumors known as lipomas. Translocation t(3;12)(q27-q28;q13-q15) with LPP is shown in lipomas. HMGA2 is also fused with a number of other genes in lipomas.
    Note=A chromosomal aberration involving HMGA2 is associated with pulmonary chondroid hamartomas. Translocation t(3;12)(q27-q28;q14-q15) with LPP is detected in pulmonary chondroid hamartomas.
    Note=A chromosomal aberration involving HMGA2 is associated with parosteal lipomas. Translocation t(3;12)(q28;q14) with LPP is also shown in one parosteal lipoma.
    Note=A chromosomal aberration involving HMGA2 is found in uterine leiomyoma. Translocation t(12;14)(q15;q23-24) with RAD51L1. Chromosomal rearrangements involving HMGA2 do not seem to be the principle pathobiological mechanism in uterine leiomyoma.
  • 序列相似性Belongs to the HMGA family.
    Contains 3 A.T hook DNA-binding domains.
  • 发展阶段Expressed predominantly during embryogenesis.
  • 翻译后修饰Regulated by cell cycle-dependent phosphorylation which alters its DNA binding affinity.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • HMGA 2 antibody
    • BABL antibody
    • High mobility group (nonhistone chromosomal) protein isoform I C antibody
    • High mobility group (nonhistone chromosomal) protein isoform IC antibody
    • High Mobility Group AT hook 2 antibody
    • High Mobility Group AT hook protein 2 antibody
    • High mobility group AT-hook protein 2 antibody
    • High mobility group protein HMGI C antibody
    • High mobility group protein HMGI-C antibody
    • High mobility group protein HMGIC antibody
    • High mobility group protein I, isoform C antibody
    • HMGA2 antibody
    • HMGA2_HUMAN antibody
    • HMGI C antibody
    • HMGIC antibody
    • LIPO antibody
    • Non histone chromosomal architectural protein HMGI C antibody
    • Non histone chromosomal architectural protein HMGIC antibody
    • Pygmy antibody
    • STQTL9 antibody
    see all

Anti-HMGA2 antibody 图像

  • Human breast carcinoma stained with Anti-HMGA2 antibody, ab52039 at 1:50 for 30 min at RT
  • ICC/IF image of ab52039 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52039, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-HMGA2 antibody (ab52039)参考文献

This product has been referenced in:
  • Wend P  et al. WNT10B/ß-catenin signalling induces HMGA2 and proliferation in metastatic triple-negative breast cancer. EMBO Mol Med : (2013). IHC-P ; Human . Read more (PubMed: 23307470) »
  • Larsson L  et al. Expression of high mobility group A proteins in oral leukoplakia. Anticancer Res 33:4261-6 (2013). IHC . Read more (PubMed: 24122990) »

See all 4 Publications for this product

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