RabMAb

Anti-HMG4抗体[EP2839Y] (ab75782)

概述

  • 产品名称Anti-HMG4抗体[EP2839Y]
    参阅全部 HMG4 一抗
  • 描述
    兔单克隆抗体[EP2839Y] to HMG4
  • 经测试应用适用于: WB, IP, IHC-P, Flow Cytmore details
    不适用于: ICC/IF
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues near the C-terminus of human HMG4

  • 阳性对照
    • 293T, HeLa and human placenta cell lysates, human placenta tissue
  • 常规说明

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

应用

Our Abpromise guarantee covers the use of ab75782 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2500 - 1/5000. Detects a band of approximately 25 kDa (predicted molecular weight: 23 kDa).
IP 1/40.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • 应用说明Is unsuitable for ICC/IF.
  • 靶标

    • 功能Binds preferentially single-stranded DNA and unwinds double stranded DNA.
    • 组织特异性Expressed predominantly in placenta.
    • 序列相似性Belongs to the HMGB family.
      Contains 2 HMG box DNA-binding domains.
    • 细胞定位Nucleus. Chromosome.
    • Information by UniProt
    • 数据库链接
    • 别名
      • chromosomal protein, Nonhistone, HMG4 antibody
      • High mobility group (nonhistone chromosomal) protein 4 antibody
      • High mobility group box 3 antibody
      • High mobility group protein 2a antibody
      • High mobility group protein 4 antibody
      • High mobility group protein B3 antibody
      • High mobility group protein HMG4 antibody
      • HMG 4 antibody
      • HMG-2a antibody
      • HMG-4 antibody
      • HMG2A antibody
      • HMGB 3 antibody
      • Hmgb3 antibody
      • HMGB3_HUMAN antibody
      • MGC90319 antibody
      • Non histone chromosomal protein antibody
      • Nonhistone chromosomal protein HMG4 antibody
      see all

    Anti-HMG4 antibody [EP2839Y] 图像

    • All lanes : Anti-HMG4 antibody [EP2839Y] (ab75782) at 1/5000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : human placenta cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti rabbit at 1/2000 dilution

      Predicted band size : 23 kDa
      Observed band size : 25 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 55 kDa (possible this band results from an immunoprecipitated sample, it is the ~55kDa heavy chain of the antibody used for the IP).
    • Immunohistochemical analysis of paraffin-embedded human placenta using ab75782 at 1/100-1/250 dilution and with the antigen retrieval step.

    Anti-HMG4 antibody [EP2839Y] (ab75782)参考文献

    ab75782 has not yet been referenced specifically in any publications.

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