Rat, Rabbit, Horse, Guinea pig, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide corresponding to a region between residues 125 and 175 of human HMG4 (NP_005333.2).
HeLa and 293T whole cell lysates.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/2000 - 1/10000. Detects a band of approximately 28 kDa (predicted molecular weight: 23 kDa).
Use at 2-5 µg/mg of lysate.
Binds preferentially single-stranded DNA and unwinds double stranded DNA.
Expressed predominantly in placenta.
Belongs to the HMGB family.
Contains 2 HMG box DNA-binding domains.
Information by UniProt
chromosomal protein, Nonhistone, HMG4 antibody
High mobility group (nonhistone chromosomal) protein 4 antibody
High mobility group box 3 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMG4 antibody (ab72544)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin basal cell carcinoma (left) and mouse squamous cell carcinoma (right) tissues labelling HMG4 with ab72544 at 1/200 (1µg/ml). Detection: DAB.
Western blot - Anti-HMG4 antibody (ab72544)
All lanes :
Anti-HMG4 antibody (ab72544) at 0.04 µg/ml
Lane 1 :
HeLa whole cell lysate at 50 µg
Lane 2 :
HeLa whole cell lysate at 15 µg
Lane 3 :
HeLa whole cell lysate at 5 µg
Lane 4 :
293T whole cell lysate at 50 µg
Predicted band size:
Observed band size:
28 kDa (
why is the actual band size different from the predicted?
Additional bands at:
65 kDa. We are unsure as to the identity of these extra bands.
Immunoprecipitation - Anti-HMG4 antibody (ab72544)
Detection of Human HMG4 by Immunoprecipitation from Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab72544 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"