概述

  • 产品名称Anti-HMG4抗体
    参阅全部 HMG4 一抗
  • 描述
    兔多克隆抗体to HMG4
  • 经测试应用适用于: IHC-P, WB, IPmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Rabbit, Horse, Guinea pig, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residues 125 and 175 of human HMG4 (NP_005333.2).

  • 阳性对照
    • HeLa and 293T whole cell lysates.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab72544 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Detects a band of approximately 28 kDa (predicted molecular weight: 23 kDa).
IP Use at 2-5 µg/mg of lysate.

靶标

  • 功能Binds preferentially single-stranded DNA and unwinds double stranded DNA.
  • 组织特异性Expressed predominantly in placenta.
  • 序列相似性Belongs to the HMGB family.
    Contains 2 HMG box DNA-binding domains.
  • 细胞定位Nucleus. Chromosome.
  • Information by UniProt
  • 数据库链接
  • 别名
    • chromosomal protein, Nonhistone, HMG4 antibody
    • High mobility group (nonhistone chromosomal) protein 4 antibody
    • High mobility group box 3 antibody
    • High mobility group protein 2a antibody
    • High mobility group protein 4 antibody
    • High mobility group protein B3 antibody
    • High mobility group protein HMG4 antibody
    • HMG 4 antibody
    • HMG-2a antibody
    • HMG-4 antibody
    • HMG2A antibody
    • HMGB 3 antibody
    • Hmgb3 antibody
    • HMGB3_HUMAN antibody
    • MGC90319 antibody
    • Non histone chromosomal protein antibody
    • Nonhistone chromosomal protein HMG4 antibody
    see all

Anti-HMG4 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin basal cell carcinoma (left) and mouse squamous cell carcinoma (right) tissues labelling HMG4 with ab72544 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-HMG4 antibody (ab72544) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg


    Predicted band size : 23 kDa
    Observed band size : 28 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 65 kDa. We are unsure as to the identity of these extra bands.
  • Detection of Human HMG4 by Immunoprecipitation from Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab72544 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.

Anti-HMG4 antibody (ab72544)参考文献

ab72544 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab72544.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"