Mouse, Rat, Cow, Dog
Synthetic peptide derived from residues 132-181 of human HMG4.
WB: placenta tissue lysate.
IHC: human intestine.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Constituents: 2% Sucrose, PBS.
Concentration information loading...
Protein A purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.3 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use at an assay dependent concentration.
Binds preferentially single-stranded DNA and unwinds double stranded DNA.
Expressed predominantly in placenta.
Belongs to the HMGB family.
Contains 2 HMG box DNA-binding domains.
Information by UniProt
chromosomal protein, Nonhistone, HMG4 antibody
High mobility group (nonhistone chromosomal) protein 4 antibody
High mobility group box 3 antibody
Anti-HMG4 antibody 图像
Western blot - HMG4 antibody (ab28727)
Anti-HMG4 antibody (ab28727) at 0.3 µg/ml + Placenta tissue lysate
Secondary HRP conjugated anti Rabbit IgG at 1/50000 dilution Developed using the ECL technique Predicted band size : 23 kDa
Immunohistochemistry (Paraffin-embedded sections) - HMG4 antibody (ab28727)
Ab28727, at a concentration of 4-8 µg/ml, staining HMG4 in paraffin embedded human intestine sections by Immunohistochemistry. Positively labelled epithelial cells of intestinal villus are indicated by arrows.
Anti-HMG4 antibody (ab28727)参考文献
has not yet been referenced specifically in any publications.
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