概述

  • 产品名称Anti-HMG14抗体
    参阅全部 HMG14 一抗
  • 描述
    兔多克隆抗体to HMG14
  • 经测试应用适用于: IP, IHC-Pmore details
    不适用于: WB
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Baboon, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 50-92 of Human HMG14 (NP_004956.5)

  • 阳性对照
    • HeLa cell lysate
  • 常规说明Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

性能

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应用

Our Abpromise guarantee covers the use of ab114127 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at 5-10 µg/mg of lysate.
IHC-P 1/100 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • 应用说明Is unsuitable for WB.
  • 靶标

    • 功能Binds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer. May be involved in the process which maintains transcribable genes in an unique chromatin conformation. Inhibits the phosphorylation of nucleosomal histones H3 and H2A by RPS6KA5/MSK1 and RPS6KA3/RSK2.
    • 序列相似性Belongs to the HMGN family.
    • 翻译后修饰Phosphorylation on Ser-21 and Ser-25 weakens binding to nucleosomes and increases the rate of H3 phosphorylation (By similarity). Phosphorylation favors cytoplasmic localization.
    • 细胞定位Nucleus. Cytoplasm. Cytoplasmic enrichment upon phosphorylation. The RNA edited version localizes to the nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • FLJ27265 antibody
      • FLJ31471 antibody
      • High mobility group (nonhistone chromosomal) protein 14 antibody
      • High mobility group nucleosome binding 1 antibody
      • High mobility group nucleosome binding domain 1 antibody
      • High mobility group nucleosome binding domain containing protein 1 antibody
      • High mobility group nucleosome-binding domain-containing protein 1 antibody
      • High mobility group protein 14 antibody
      • HMG 14 antibody
      • HMG14 antibody
      • HMGN 1 antibody
      • HMGN1 antibody
      • HMGN1_HUMAN antibody
      • MGC104230 antibody
      • MGC117425 antibody
      • Non-histone chromosomal protein HMG-14 antibody
      • Nonhistone chromosomal protein HMG 14 antibody
      • Nonhistone chromosomal protein HMG14 antibody
      see all

    Anti-HMG14 antibody 图像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling HMG14 with ab114127 at 1/1000 (1µg/ml). Detection: DAB.
    • ab114127 at 10ug/mg of lysate detecting HMG14 by immunoprecipitation (lane 1). 1mg of HeLa whole cell lysate was used, 20% of immunoprecipitate was loaded. Lane 2: IgG control.
      Detection: chemiluminescence. Exposure time: 30 seconds.

    Anti-HMG14 antibody (ab114127)参考文献

    ab114127 has not yet been referenced specifically in any publications.

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