概述

  • 产品名称Anti-HLTF抗体
    参阅全部 HLTF 一抗
  • 描述
    山羊多克隆抗体to HLTF
  • 特异性This antibody is expected to recognise both human isoforms of this protein.
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide:

    C-KQAKINEIRTLIDL

    , corresponding to C terminal amino acids 996-1009 of Human HLTF(Peptide available as ab22937.)

  • 阳性对照
    • Jurkat lysate.
  • 常规说明Principal Names – SMARCA3; HLTF; ZBU1; HLTF1; HIP116; SNF2L3; HIP116A; SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 3; SNF2-like 3; sucrose nonfermenting-like 3; helicase-like transcription factor; DNA-binding protein/plasminogen activator inhibitor-1 regulator. Official Gene Symbol - SMARCA3. GenBank Accession Number – NP_003062; NP_620636. LocusLink ID - 6596 (human); 20585 (mouse). Gene Ontology terms - adenosinetriphosphatase; DNA binding; regulation of transcription, DNA-dependent; nucleus.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab2835 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明Western Blot: Approx 120kDa and 50kDa bands seen in Jurkat cell lysate.
    Recommended for use at 0.2-1.0ug/ml (predicted molecular weight: 114 kDa).

    Please note that the weeker smaller band may represent a novel isoform of this protein and is in agreement with previous Western blot observations (J Biol Chem 1995 Mar 3;270(9):4575-87).
  • 靶标

    • 功能Has both helicase and E3 ubiquitin ligase activities. Possesses intrinsic ATP-dependent nucleosome-remodeling activity; This activity may be required for transcriptional activation or repression of specific target promoters (By similarity). These may include the SERPINE1 and HIV-1 promoters and the SV40 enhancer, to which this protein can bind directly. Plays a role in error-free postreplication repair (PRR) of damaged DNA and maintains genomic stability through acting as a ubiquitin ligase for 'Lys-63'-linked polyubiquitination of chromatin-bound PCNA.
    • 组织特异性Expressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle.
    • 通路Protein modification; protein ubiquitination.
    • 序列相似性Belongs to the SNF2/RAD54 helicase family. RAD16 subfamily.
      Contains 1 helicase ATP-binding domain.
      Contains 1 helicase C-terminal domain.
      Contains 1 RING-type zinc finger.
    • 细胞定位Cytoplasm. Nucleus. Nucleus, nucleolus. Nucleus, nucleoplasm. Nuclear localization is stimulated by progesterone.
    • Information by UniProt
    • 数据库链接
    • 别名
      • DNA-binding protein/plasminogen activator inhibitor 1 regulator antibody
      • Helicase like transcription factor antibody
      • Helicase-like transcription factor antibody
      • HIP116 antibody
      • HIP116A antibody
      • HLTF 1 antibody
      • Hltf antibody
      • HLTF_HUMAN antibody
      • HLTF1 antibody
      • p113 antibody
      • RING finger protein 80 antibody
      • RNF80 antibody
      • SMARC A3 antibody
      • SMARCA 3 antibody
      • SMARCA3 antibody
      • SNF2-like 3 antibody
      • SNF2L3 antibody
      • Sucrose nonfermenting protein 2 like 3 antibody
      • Sucrose nonfermenting protein 2-like 3 antibody
      • SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily A member 3 antibody
      • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin a3 antibody
      • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 3 antibody
      • ZBU 1 antibody
      • ZBU1 antibody
      see all

    Anti-HLTF antibody 图像



    • Predicted band size : 114 kDa


      ab2835 staining (0.5µg/ml) of Jurkat lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

    Anti-HLTF antibody (ab2835)参考文献

    ab2835 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab2835.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"