Anti-HLA G抗体[MEM-G/9] (ab7758)

概述

  • 产品名称Anti-HLA G抗体[MEM-G/9]
    参阅全部 HLA G 一抗
  • 描述
    小鼠单克隆抗体[MEM-G/9] to HLA G
  • 特异性Reacts with the native form of human HLA-G on the cell surface as well as with soluble HLA-G molecule in its beta 2-microglobulin associated form. Specificity was demonstrated with cells transfected with either full-length HLA-G and HLA-G1 complementary DNA. This antibody does not compete with a tested panel of other monoclonal antibodies to HLA-G. We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • 经测试应用适用于: ICC/IF, IHC-Fr, WB, ELISA, Flow Cytmore details
    不适用于: IHC-P
  • 种属反应性
    与反应: Human
    预测可用于: Non Human Primates
  • 免疫原

    Recombinant full length protein (Human).

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 15mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 纯度>95% by SDS-PAGE
  • 纯化说明Purified from ascites using protein A affinity chromatography.
  • 克隆单克隆
  • 克隆编号MEM-G/9
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab7758 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.

Do not fix cells, see Menier et al.

IHC-Fr Use at an assay dependent concentration.
WB 1/2000. The antibody MEM-G/9 reacts both with cell surface and soluble form of HLA-G, but is not suitable for standard WB as it detects native antigen.
ELISA 1/100 - 1/2500. See Abreview.
Flow Cyt Use at an assay dependent concentration. Do not fix cells, see Menier et al.



ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
  • 应用说明Is unsuitable for IHC-P.
  • 靶标

    • 功能Involved in the presentation of foreign antigens to the immune system. Plays a role in maternal tolerance of the fetus by mediating protection from the deleterious effects of natural killer cells, cytotoxic T lymphocytes, macrophages and mononuclear cells.
    • 组织特异性Expressed in trophoblasts.
    • 序列相似性Belongs to the MHC class I family.
      Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
    • 细胞定位Membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • B2 microglobulin antibody
      • DADB-15K14.8 antibody
      • HLA 6.0 antibody
      • HLA class I histocompatibility antigen alpha chain G antibody
      • HLA class I histocompatibility antigen, alpha chain G antibody
      • HLA class I molecule antibody
      • HLA G antibody
      • HLA G antigen antibody
      • HLA G histocompatibility antigen class I G antibody
      • HLA G3 antibody
      • HLA-G antibody
      • HLA-G histocompatibility antigen, class I antibody
      • HLA60 antibody
      • HLAG antibody
      • HLAG_HUMAN antibody
      • Major histocompatibility complex class I G antibody
      • MHC class I antigen antibody
      • MHC class I antigen G antibody
      • MHC G antibody
      • T-cell A locus antibody
      • TCA antibody
      see all

    Anti-HLA G antibody [MEM-G/9] 图像

    • ab7758 staining HLA G in Human JEG-3 cells by Flow Cytometry. The sample was incubated with the primary antibody, (1/50), for 1 hour. Ab6785(1/100) was used as secondary antibody.
      Gating Strategy: Isotrope control

      See Abreview

    • ab7758 staining HLA G in Human JEG-3 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were blocked with 10% serum for 45 minutes at 24°C. Samples were incubated with primary antibody (1/50) for 1 hour at 24°C. Ab6785 (1/100) was used as the secondary antibody.

      See Abreview

    Anti-HLA G antibody [MEM-G/9] (ab7758)参考文献

    This product has been referenced in:
    • Le Page ME  et al. Killer Ig-like receptor 2DL4 does not mediate NK cell IFN-? responses to soluble HLA-G preparations. J Immunol 192:732-40 (2014). Read more (PubMed: 24337374) »
    • Heidenreich S  et al. Impact of the NK cell receptor LIR-1 (ILT-2/CD85j/LILRB1) on cytotoxicity against multiple myeloma. Clin Dev Immunol 2012:652130 (2012). Flow Cyt ; Human . Read more (PubMed: 22844324) »

    See all 9 Publications for this product

    Product Wall

    Thank you for contacting us.

    The antibody is conjugated with neither Fluorescence dye. If this would be the case we would have mentioned this within the name eg. ab7758 Anti-HLA G antibody [MEM-G/9] (PE) and further in the short descriptio...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (Jeg-3 cell line)
    Loading amount 100 µg
    Specification Jeg-3 cell line
    Gel Running Conditions Reduced Denaturing (10)
    Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Username

    Abcam user community

    Verified customer

    提交于 Apr 20 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (JEG-3)
    Specification JEG-3
    Fixative NONE
    Permeabilization No
    Blocking step Serum as blocking agent for 45 minute(s) · Concentration: 10% · Temperature: 24°C
    Username

    Abcam user community

    Verified customer

    提交于 Apr 21 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Flow Cytometry
    Sample Human Cell (JEG-3)
    Specification JEG-3
    Fixation NONE
    Permeabilization No
    Gating Strategy isotype control
    Username

    Abcam user community

    Verified customer

    提交于 Apr 20 2010

    Unfortunately, no information on the reactivity with G*01012 or G*01013 alleles is available.

    Probably not as the cross reactivity is restricted to primates.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"