概述

  • 产品名称Anti-HLA G抗体[G233]
    参阅全部 HLA G 一抗
  • 描述
    小鼠单克隆抗体[G233] to HLA G
  • 特异性ab52454 recognises several isoforms of HLA-G expressed in all populations of extravillous trophoblast (cell columns, interstitial trophoblast, endovascular trophoblast, placental bed giant cells). It does not cross-react with any other MHC Class I antigens (HLA-A, -B, -C, -E, -F).
  • 经测试应用适用于: ICC/IF, IP, ELISA, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Murine L cells transfected with both human beta2-microglobulin and HLA-G.

  • 阳性对照
    • Jeg3 cell line.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 15mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 纯度>95% by SDS-PAGE
  • 纯化说明Purified from hybridoma culture supernatant by protein-A affinity chromatography.
  • 克隆单克隆
  • 克隆编号G233
  • 同种型IgG2a
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab52454 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 10 µg/ml.
IP Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
Flow Cyt Use a concentration of 5 - 10 µg/ml. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Involved in the presentation of foreign antigens to the immune system. Plays a role in maternal tolerance of the fetus by mediating protection from the deleterious effects of natural killer cells, cytotoxic T lymphocytes, macrophages and mononuclear cells.
  • 组织特异性Expressed in trophoblasts.
  • 序列相似性Belongs to the MHC class I family.
    Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • B2 microglobulin antibody
    • DADB-15K14.8 antibody
    • HLA 6.0 antibody
    • HLA class I histocompatibility antigen alpha chain G antibody
    • HLA class I histocompatibility antigen, alpha chain G antibody
    • HLA class I molecule antibody
    • HLA G antibody
    • HLA G antigen antibody
    • HLA G histocompatibility antigen class I G antibody
    • HLA G3 antibody
    • HLA-G antibody
    • HLA-G histocompatibility antigen, class I antibody
    • HLA60 antibody
    • HLAG antibody
    • HLAG_HUMAN antibody
    • Major histocompatibility complex class I G antibody
    • MHC class I antigen antibody
    • MHC class I antigen G antibody
    • MHC G antibody
    • T-cell A locus antibody
    • TCA antibody
    see all

Anti-HLA G antibody [G233] 图像

  • ICC/IF image of ab52454 stained Jeg3 cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52454, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-HLA G antibody [G233] (ab52454)参考文献

This product has been referenced in:
  • Cho S  et al. The C-terminal amino acid of the MHC-I heavy chain is critical for binding to Derlin-1 in human cytomegalovirus US11-induced MHC-I degradation. PLoS One 8:e72356 (2013). Human . Read more (PubMed: 23951315) »
  • Gonzalez A  et al. Identification of circulating nonclassic human leukocyte antigen G (HLA-G)-like molecules in exudates. Clin Chem 57:1013-22 (2011). Read more (PubMed: 21527645) »

See all 6 Publications for this product

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