Anti-HLA G抗体[5A6G7] (ab76869)

概述

  • 产品名称Anti-HLA G抗体[5A6G7]
    参阅全部 HLA G 一抗
  • 描述
    小鼠单克隆抗体[5A6G7] to HLA G
  • 经测试应用适用于: ELISA, WB, IHC-P, IHC-Fr, ICC, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Other Immunogen Type corresponding to HLA G. C-terminal amino acid sequence (22-mer) of soluble HLA-G5 and HLA-G6 protein coupled to ovalbumin

  • 阳性对照
    • JEG3 cell lysate; HeLa/ M8 - HLA G5/6 transfected cell lysates, HeLa/HLA-G5 cell supernatant; placenta tissue.
  • 常规说明


    ab76869 allows discrimination between soluble HLA-G protein yielded by shedding from membrane-bound HLA-G forms that do not contain the intron 4-encoded epitope and soluble HLA-G5/HLA-G6 produced from intron 4-retaining alternatively spliced mRNAs.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 15mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 纯度>95% by SDS-PAGE
  • 纯化说明Purified from ascites by precipitation methods.
  • Primary antibody说明ab76869 allows discrimination between soluble HLA-G protein yielded by shedding from membrane-bound HLA-G forms that do not contain the intron 4-encoded epitope and soluble HLA-G5/HLA-G6 produced from intron 4-retaining alternatively spliced mRNAs.
  • 克隆单克隆
  • 克隆编号5A6G7
  • 同种型IgG1
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab76869 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration. Can be as capture antibody to analyze of soluble HLA G together with a suitable detection antibody.
WB Use at an assay dependent concentration. Predicted molecular weight: 38 kDa.
IHC-P Use a concentration of 10 - 20 µg/ml.
IHC-Fr Use a concentration of 10 - 20 µg/ml.
ICC Use at an assay dependent concentration.
Flow Cyt Use a concentration of 2 µg/ml. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Involved in the presentation of foreign antigens to the immune system. Plays a role in maternal tolerance of the fetus by mediating protection from the deleterious effects of natural killer cells, cytotoxic T lymphocytes, macrophages and mononuclear cells.
  • 组织特异性Expressed in trophoblasts.
  • 序列相似性Belongs to the MHC class I family.
    Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • B2 microglobulin antibody
    • DADB-15K14.8 antibody
    • HLA 6.0 antibody
    • HLA class I histocompatibility antigen alpha chain G antibody
    • HLA class I histocompatibility antigen, alpha chain G antibody
    • HLA class I molecule antibody
    • HLA G antibody
    • HLA G antigen antibody
    • HLA G histocompatibility antigen class I G antibody
    • HLA G3 antibody
    • HLA-G antibody
    • HLA-G histocompatibility antigen, class I antibody
    • HLA60 antibody
    • HLAG antibody
    • HLAG_HUMAN antibody
    • Major histocompatibility complex class I G antibody
    • MHC class I antigen antibody
    • MHC class I antigen G antibody
    • MHC G antibody
    • T-cell A locus antibody
    • TCA antibody
    see all

Anti-HLA G antibody [5A6G7] 图像

  • ab76869 staining HLA G in human placenta tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a TRIS-EDTA Buffer. Samples were incubated with primary antibody (1/50 in blocking buffer) for 30 minutes at 20°C. A HRP-conjugated goat anti-mouse IgG polyclonal was used as the secondary antibody.

    See Abreview

  • All lanes : Anti-HLA G antibody [5A6G7] (ab76869)

    Lane 1 : M8 cell line transfected with empty vector
    Lane 2 : M8 cell line transfected with HLA G1
    Lane 3 : M8 cell line transfected with HLA G5
    Lane 4 : M8 cell line transfected with HLA G6


    Predicted band size : 38 kDa
    Observed band size : 30,38 kDa (why is the actual band size different from the predicted?)

Anti-HLA G antibody [5A6G7] (ab76869)参考文献

This product has been referenced in:
  • Du MR  et al. Embryonic trophoblasts induce decidual regulatory T cell differentiation and maternal-fetal tolerance through thymic stromal lymphopoietin instructing dendritic cells. J Immunol 192:1502-11 (2014). Flow Cyt ; Human . Read more (PubMed: 24453244) »
  • Guo Y  et al. Soluble human leukocyte antigen-g5 activates extracellular signal-regulated protein kinase signaling and stimulates trophoblast invasion. PLoS One 8:e76023 (2013). WB ; Human . Read more (PubMed: 24098421) »

See all 6 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Placenta)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: TRIS-EDTA Buffer pH 9,0
Permeabilization Yes - Wash Buffer from Dako with Tween
Specification Placenta
Fixative Paraformaldehyde
Username

Mr. Rudolf Jung

Verified customer

提交于 Mar 09 2016

Merci de votre appel de toutà l'heure.

Voici les résultats du blast promises. Comme discuté au téléphone, nous avons aussi des anticorps contre le RT1 class Ib de rat directement, comme par exemple le ab3374...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"