Anti-HLA G抗体[4H84] (ab52455)

概述

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 15mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 纯度
    >95% by SDS-PAGE
  • 纯化说明
    Purified from hybridoma culture supernatant by protein-A affinity chromatography.
  • 克隆
    单克隆
  • 克隆编号
    4H84
  • 同种型
    IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab52455 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 38 kDa.
IP Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能
    Involved in the presentation of foreign antigens to the immune system. Plays a role in maternal tolerance of the fetus by mediating protection from the deleterious effects of natural killer cells, cytotoxic T lymphocytes, macrophages and mononuclear cells.
  • 组织特异性
    Expressed in trophoblasts.
  • 序列相似性
    Belongs to the MHC class I family.
    Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • B2 microglobulin antibody
    • DADB-15K14.8 antibody
    • HLA 6.0 antibody
    • HLA class I histocompatibility antigen alpha chain G antibody
    • HLA class I histocompatibility antigen, alpha chain G antibody
    • HLA class I molecule antibody
    • HLA G antibody
    • HLA G antigen antibody
    • HLA G histocompatibility antigen class I G antibody
    • HLA G3 antibody
    • HLA-G antibody
    • HLA-G histocompatibility antigen, class I antibody
    • HLA60 antibody
    • HLAG antibody
    • HLAG_HUMAN antibody
    • Major histocompatibility complex class I G antibody
    • MHC class I antigen antibody
    • MHC class I antigen G antibody
    • MHC G antibody
    • T-cell A locus antibody
    • TCA antibody
    see all

图片

  • ab52455 staining HLA G in Human spleen tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and permeabilized Wash Buffer with Tween; antigen retrieval was by heat mediation in a TRIS-EDTA Buffer (pH 9.0). Samples were incubated with primary antibody (1/50 in diluent) for 30 minutes at 20°C. An undiluted Goat anti-Mouse HRP polyclonal was used as the secondary antibody.

    See Abreview

文献

This product has been referenced in:
  • Jasinski-Bergner S  et al. Clinical relevance of miR-mediated HLA-G regulation and the associated immune cell infiltration in renal cell carcinoma. Oncoimmunology 4:e1008805 (2015). Read more (PubMed: 26155421) »
  • Lim R  et al. Preterm human amnion epithelial cells have limited reparative potential. Placenta 34:486-92 (2013). Read more (PubMed: 23597502) »

See all 6 Publications for this product

客户评价及客户问答

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Human Tissue sections (Human placental explants)
Antigen retrieval step
None
Permeabilization
No
Specification
Human placental explants
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Dr. Angela Crespo

Verified customer

提交于 Sep 15 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (First trimester decidua)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: sodium citrate
Permeabilization
No
Specification
First trimester decidua
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Formaldehyde
Username

Ruhul Choudhury

Verified customer

提交于 Jan 24 2017

Application
Western blot
Sample
Human Cell lysate - whole cell (NPC)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
NPC
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Jan 09 2017

Merci de votre appel de toutà l'heure.

Voici les résultats du blast promises. Comme discuté au téléphone, nous avons aussi des anticorps contre le RT1 class Ib de rat directement, comme par exemple le ab3374...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (spleen)
Specification
spleen
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: TRIS-EDTA Buffer (pH 9.0)
Permeabilization
Yes - DAKO Wash-Buffer with Tween
Username

Mr. Rudolf Jung

Verified customer

提交于 May 01 2012

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has ca...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (JEG-3)
Specification
JEG-3
Fixation
None
Permeabilization
No
Gating Strategy
iostype control
Username

Abcam user community

Verified customer

提交于 May 17 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (JEG-3)
Loading amount
30 µg
Specification
JEG-3
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
Username

Abcam user community

Verified customer

提交于 May 05 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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