概述

  • 产品名称Anti-HLA F抗体
    参阅全部 HLA F 一抗
  • 描述
    兔多克隆抗体to HLA F
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Cow, Pig, Chimpanzee, African Green Monkey
  • 免疫原

    A synthetic peptide corresponding to a region of human HLA F

  • 阳性对照
    • 721_B cell lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Purified by peptide affinity chromatography method.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab81070 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/100.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 39 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Involved in the presentation of foreign antigens to the immune system.
  • 序列相似性Belongs to the MHC class I family.
    Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CDA12 antibody
    • HLA 5.4 antibody
    • HLA class I histocompatibility antigen, alpha chain F antibody
    • HLA class I molecule antibody
    • HLA F antigen antibody
    • HLA-CDA12 antibody
    • HLA-F antibody
    • HLAF antibody
    • HLAF_HUMAN antibody
    • Leukocyte antigen F antibody
    • MHC class I antigen F antibody
    see all

Anti-HLA F antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labelling HLA F with ab81070 at 1/100. A Cy3-conjugated donkey anti-rabbit IgG (1/200) was used as the secondary antibody. Positive staining shown in the membrane and cytoplasm of alveolar type I cells. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - HLA F. Right - Merge.
  • Anti-HLA F antibody (ab81070) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 39 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)

Anti-HLA F antibody (ab81070)参考文献

ab81070 has not yet been referenced specifically in any publications.

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