Anti-HLA E抗体[MEM-E/08] (ab11821)

概述

  • 产品名称
    Anti-HLA E抗体[MEM-E/08]
    参阅全部 HLA E 一抗
  • 描述
    小鼠单克隆抗体[MEM-E/08] to HLA E
  • 特异性
    The antibody recognized native surface-expressed HLA E, but not denaturated heavy chain of HLA E. The published results revealed that antibody is remarkably specific for HLA E, only with weak cross-reactivity with following classical MHC Class I molecules: HLA A24, B7, B27, B51, B54, C7.
  • 经测试应用
    适用于: WB, Flow Cyt, IPmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Bacterially expressed recombinant HLA-E refolded with beta2-microglobulin and peptide.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 15mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 纯度
    Protein A purified
  • 纯化说明
    Purified from hybridoma culture supernatant by protein A-affinity chromatography.
  • 克隆
    单克隆
  • 克隆编号
    MEM-E/08
  • 同种型
    IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab11821 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
Flow Cyt Use a concentration of 4 µg/ml. Tested on HLA-E transfected human cell line LCL 721.221



ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IP Use at an assay dependent concentration.

靶标

  • 相关性
    HLA E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA E binds a restricted subset of peptides derived from the leader peptides of other class I molecules.
  • 细胞定位
    Membrane; Single-pass type I membrane protein
  • 数据库链接
  • 别名
    • HLA class I histocompatibility antigen E alpha chain antibody
    • EA1.2 antibody
    • EA2.1 antibody
    • HLA 6.2 antibody
    • HLA class I histocompatibility antigen alpha chain E antibody
    • HLA class I histocompatibility antigen E alpha chain precursor antibody
    • HLA class I histocompatibility antigen E alpha chain precursor antibody
    • HLA6.2 antibody
    • HLAE antibody
    • Lymphocyte antigen antibody
    • Major histocompatibility complex class I E antibody
    • MHC antibody
    • MHC class I antigen E antibody
    • MHC HLA E alpha 1 antibody
    • MHC HLA E alpha 2.1 antibody
    • QA1 antibody
    see all

图片

  • ab11821 staining HLA E in Human K562 cells by Flow Cytometry. The sample was incubated with the primary antibody, (1/50), for 1 hour at 24°C. Ab6785(1/100) was used as secondary antibody.
    Gating Strategy: Isotrope control

    See Abreview

  • Anti-HLA E antibody [MEM-E/08] (ab11821) at 1 µg/ml + Human spleen tissue lysate - total protein (ab29699) at 10 µg

    Secondary
    Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 40 kDa
    Observed band size : 40 kDa
    Additional bands at : 50 kDa,68 kDa,73 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes

文献

This product has been referenced in:
  • Wang F  et al. S6K-STING interaction regulates cytosolic DNA-mediated activation of the transcription factor IRF3. Nat Immunol 17:514-522 (2016). WB, IP ; Mouse . Read more (PubMed: 27043414) »
  • Boura JS  et al. Evaluation of gene delivery strategies to efficiently overexpress functional HLA-G on human bone marrow stromal cells. Mol Ther Methods Clin Dev 2014:N/A (2014). Flow Cyt ; Human . Read more (PubMed: 25279386) »

See all 7 Publications for this product

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (K562)
Specification
K562
Fixation
NONE
Permeabilization
No
Gating Strategy
iostype control
Username

Abcam user community

Verified customer

提交于 Apr 20 2010

Thank you for your patience in this matter. The originator of ab11821 (HLA E antibody [MEM-E/08]) has provided the following references: Sibilio L. et al., Tissue Antigens 62, 339, 2003. (Abstract 45 from the 3rd Int. Conference on HLA-G, Paris...

Read More

This antibody was tested on the human cell line LCL 721.221 (HLA-E transfected), dilution 1:50 - 1:500. This antibody reacts with the native molecule of HLA-E. I hope this helps. Please contact us again if you have any additional questions.

Thank you for your enquiry. This antibody perfectly works in flow cytometry but as far as we know, this antibody was not tested on lymphocytes. It was tested in FACS with HLA-E transfected human cell line LCL 721.221. Have you tried titrating ...

Read More

Thank you for your equiry. For testing this antibody on FACS was used human cell line LCL 721.221, HLA-E transfected. Please do not hesitate to contact us if you need anything further.

Thank you for your enquiry and your interest in our products. This antibody perfectly works in flow cytometry but we have not used HeLa cell line for this study. Perhaps this information can be found in some published paper.

The antibody appears to be very specific and most likely will not reacts with the allele of question. We will update our on-line datasheet with the cross-reactivity results very soon.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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