概述

  • 产品名称Anti-HLA E抗体[MEM-E/06]
    参阅全部 HLA E 一抗
  • 描述
    小鼠单克隆抗体[MEM-E/06] to HLA E
  • 特异性The antibody recognized native surface-expressed HLA E, but not denaturated heavy chain of HLA E. The published results showed that the antibody cross-reacts with some classical MHC Class I molecules (HLA A3, A11, B7). However, the recent Workshop I Session on the 3rd International Conference on HLA-G (Paris, July 2003) confirmed that the antibody exhibits much broader cross-reactivity classical MHC Class I antigens, namely with HLA A24, A32, B8, B15, B27, B35, B44, B54, C3, C4, C5 and C7.
  • 经测试应用适用于: Flow Cyt, IP, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length protein corresponding to Human HLA E.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 15mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 纯度>95% by SDS-PAGE
  • 纯化说明Purified from ascites by protein A affinity chromatography.
  • 克隆单克隆
  • 克隆编号MEM-E/06
  • 骨髓瘤unknown
  • 同种型IgG1
  • 轻链类型unknown
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab3984 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
IHC-P Use a concentration of 10 µg/ml.

靶标

  • 相关性HLA E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA E binds a restricted subset of peptides derived from the leader peptides of other class I molecules.
  • 细胞定位Membrane; Single-pass type I membrane protein
  • 数据库链接
  • 别名
    • HLA class I histocompatibility antigen E alpha chain antibody
    • EA1.2 antibody
    • EA2.1 antibody
    • HLA 6.2 antibody
    • HLA class I histocompatibility antigen alpha chain E antibody
    • HLA class I histocompatibility antigen E alpha chain precursor antibody
    • HLA class I histocompatibility antigen E alpha chain precursor antibody
    • HLA6.2 antibody
    • HLAE antibody
    • Lymphocyte antigen antibody
    • Major histocompatibility complex class I E antibody
    • MHC antibody
    • MHC class I antigen E antibody
    • MHC HLA E alpha 1 antibody
    • MHC HLA E alpha 2.1 antibody
    • QA1 antibody
    see all

Anti-HLA E antibody [MEM-E/06] (ab3984)参考文献

This product has been referenced in:
  • Zhao L  et al. Heterelogous expression of mutated HLA-G decreases immunogenicity of human embryonic stem cells and their epidermal derivatives. Stem Cell Res 13:342-54 (2014). Flow Cyt ; Human . Read more (PubMed: 25218797) »
  • Menier C  et al. Characterization of monoclonal antibodies recognizing HLA-G or HLA-E: new tools to analyze the expression of nonclassical HLA class I molecules. Hum Immunol 64:315-26 (2003). Read more (PubMed: 12590976) »

See all 2 Publications for this product

Product Wall

Thank you for your enquiry. We have sold several vials of this product and do not have any complaint. This is a very specific question. We would suggest, (together with FACS analysis) to lyse the same cells in native lysis buffer (1%NP-40 plus inhi...

Read More

Thank you for your enquiry. We have sold several vials of this product and do not have any complaint. This is a very specific question. We would suggest, (together with FACS analysis) to lyse the same cells in native lysis buffer (1%NP-40 plus inhi...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"