概述

  • 产品名称Anti-HLA DRB5抗体[MRC OX-17]
    参阅全部 HLA DRB5 一抗
  • 描述
    小鼠单克隆抗体[MRC OX-17] to HLA DRB5
  • 特异性Does not bind to thymocytes or erythrocytes.
    Does not cross-react with rat Ia-A or mouse Ia-E antigen, but rabbit antibody raised against the antibody affinity column-purified MRC OX-17 antigen cross-reacted on tissues of mice expressing Ia-E mouse antigen but not on those mouse strains that were Ia-E antigen negative.
  • 经测试应用适用于: Flow Cytmore details
  • 种属反应性
    与反应: Rat
  • 免疫原

    Rat spleen membrane glycoproteins depleted of Ia-A antigens.

  • 阳性对照
    • Thymus (12% positive cells); Spleen (34% positive cells); Lymph Node (25% positive cells). Wistar, Buffalo, Brown Norway, ACI, and Fischer 344 stains.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液Preservative: 0.02% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • 纯度Protein G purified
  • 克隆单克隆
  • 克隆编号MRC OX-17
  • 骨髓瘤x63-Ag8.653
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab119795 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt 1/250 - 1/500. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accomodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form an heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal miroenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading.
  • 序列相似性Belongs to the MHC class II family.
    Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
  • 翻译后修饰Ubiquitinated by MARCH1 and MARCH8 at Lys-254 leading to down-regulation of MHC class II.
  • 细胞定位Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus > trans-Golgi network membrane. Endosome membrane. Lysosome membrane. Late endosome membrane. The MHC class II complex transits through a number of intracellular compartments in the endocytic pathway until it reaches the cell membrane for antigen presentation.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DR 9 antibody
    • DR beta 5 antibody
    • DR beta 5 chain antibody
    • DR beta-5 antibody
    • DR beta5 antibody
    • DR2 beta 2 antibody
    • DR2-beta-2 antibody
    • DR9 antibody
    • DRB5_HUMAN antibody
    • Dw2 antibody
    • FLJ76359 antibody
    • HLA class II histocompatibility antigen antibody
    • HLA class II histocompatibility antigen DR beta 5 chain antibody
    • HLA class II histocompatibility antigen DRB1-9 beta chain antibody
    • HLA class II histocompatibility antigen, DR-5 beta chain antibody
    • HLA DRB antibody
    • HLA-DRB5 antibody
    • Human leucocyte antigen DRB5 antibody
    • Leukocyte antigen class II antibody
    • Major histocompatibility complex, class II, DR beta 51 antibody
    • MHC class II antigen DRB1*9 antibody
    • MHC class II antigen DRB5 antibody
    • MHC class II HLA beta chain antibody
    • MHC HLA-DR-beta cell surface glycoprotein antibody
    • MHC HLA-DR-beta chain antibody
    see all

Anti-HLA DRB5 antibody [MRC OX-17] 图像

  • Rat Strain: Lewis Rat
    Cell Concentration: 1x106 cells
    Antibody Concentration: 1:400
    Isotypic Control: Mouse IgG , k
    Cell Source: Splenocytes
    Percentage of Cells Stained Above Control: 34% .

Anti-HLA DRB5 antibody [MRC OX-17] (ab119795)参考文献

This product has been referenced in:
  • Fukumoto T  et al. Mouse monoclonal antibodies against rat major histocompatibility antigens. Two Ia antigens and expression of Ia and class I antigens in rat thymus. Eur J Immunol 12:237-43 (1982). Read more (PubMed: 6178598) »

See 1 Publication for this product

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