Anti-HLA DR + HLA DP抗体[HL-40] (ab8085)

概述

  • 产品名称Anti-HLA DR + HLA DP抗体[HL-40]
    参阅全部 HLA DR + HLA DP 一抗
  • 描述
    小鼠单克隆抗体[HL-40] to HLA DR + HLA DP
  • 特异性Common epitope on b-chain of human HLA-DR and HLA-DP. Also reacts with porcine MHC class II antigens (SLA-D gene products).
  • 经测试应用适用于: WB, Flow Cyt, ICC/IF, IPmore details
  • 种属反应性
    与反应: Human, Pig
  • 免疫原

    Burkitt lymphoma cell line Raji.

  • 常规说明


    The antibody is useful for detection of HLA-DR and HLA-DP positive B-cells, T-cells and monocytes in cell suspensions, in frozen sections as well as in formalin fixed paraffin embedded tissue sections. The antibody is also useful for characterization of leukemias and lymphomas. In experimental settings it can be used for selective removal of antigen carrying cells by complement mediated cytotoxicity. The antibody has excellent staining properties in histology.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 15mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 纯度>95% by SDS-PAGE
  • 纯化说明Purified from ascites by protein-A affinity chromatography.
  • Primary antibody说明The antibody is useful for detection of HLA-DR and HLA-DP positive B-cells, T-cells and monocytes in cell suspensions, in frozen sections as well as in formalin fixed paraffin embedded tissue sections. The antibody is also useful for characterization of leukemias and lymphomas. In experimental settings it can be used for selective removal of antigen carrying cells by complement mediated cytotoxicity. The antibody has excellent staining properties in histology.
  • 克隆单克隆
  • 克隆编号HL-40
  • 同种型IgG2a
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab8085 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. PubMed: 19862823
Flow Cyt Use 0.01µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration. PubMed: 20959457
IP Use at an assay dependent concentration.

靶标

  • 相关性Human MHC class II antigens are transmembrane glycoproteins composed of an alpha (36 kDa) and a beta chain (27kDa) and are expressed primarily on antigen presenting cells. Human MHC class II genes are located in the HLA-D region that encodes at least 6 alpha and 10 beta chain genes. Three loci, DR, DQ and DP, encode the major products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides and present them to T-helper cells and therefore have a critical role in the initiation of the immune response. It has been shown that some autoimmune diseases are associated with certain class II alleles.
  • 细胞定位Cell Membrane; Single pass type I membrane protein
  • 数据库链接
  • 别名
    • HLA DP antibody
    • HLA DR antibody
    • MHC Class II antibody

Anti-HLA DR + HLA DP antibody [HL-40] 图像

  • Human peripheral blood lymphocytes stained with ab8085 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4°C. Cells were then incubated with the antibody (ab8085, 0.01μg/1x106 cells) for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 4°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Gating strategy - peripheral blood lymphocytes.

  • HeLa cells were transfected with DR. The cells were fixed in paraformaldehyde and either left unpermeabilized (-Tx) or were permeabilized with 0.2% Triton X-100 (+Tx). DR- and DP-transfected cells were immunostained with ab8085. Nuclei were stained with DAPI.
  • HeLa cells were transfected with different combinations of a, ß, and Ii constructs as indicated. Lysates were first subjected to immunoprecipitation (IP) with ab8085 before Western blotting (WB) with anti-DP and anti-Ii. H and L indicate the heavy and light chains of the antibodies used for the immunoprecipitation.

Anti-HLA DR + HLA DP antibody [HL-40] (ab8085)参考文献

This product has been referenced in:
  • van Lith M  et al. HLA-DP, HLA-DQ, and HLA-DR Have Different Requirements for Invariant Chain and HLA-DM. J Biol Chem 285:40800-8 (2010). ICC/IF, IP ; Human . Read more (PubMed: 20959457) »
  • Degenhardt Y  et al. Distinct MHC gene expression patterns during progression of melanoma. Genes Chromosomes Cancer 49:144-54 (2010). WB ; Human . Read more (PubMed: 19862823) »

See all 5 Publications for this product

Product Wall

Thank you for your enquiry. This antibody has not been tested for cross-reaction with sheep(ovine) antigens.

Yes WB was performed under reducing conditions. For most details on Western Blotting, please refer to Horejsi et al. Tissue Antigens 28, 288-291 (1986)

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"