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Tissue, cells or virus corresponding to Human HLA DR + DP + DQ. Raised against tonsil cells of human origin
This antibody principally labels B cells, interdigitating reticulum cells, Langerhans’ cells and manymacrophages.
Our Abpromise guarantee covers the use of ab7856 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|IP||Use at an assay dependent concentration.|
|WB||1/500. Detects a band of approximately 30 kDa (predicted molecular weight: 27 kDa).|
Ab7856 staining HLA DR+DP+DQ in Human Colorectal tissue sections by Immunohistochemistry. Antigen retrieval was by heat mediation at pH6 for 20minutes. Samples were fixed with formaldehyde and blocked with 10% hydrogen peroxide for 5 minutes. Samples were incubated with primary antibody at 1/100 dilution for 15 minutes. HRP rabbit polyclonal polymer refine detection kit was used as the secondary antibody.