Anti-HLA DR抗体[GRB-1] (PerCP) (ab91333)

概述

  • 产品名称Anti-HLA DR抗体[GRB-1] (PerCP)
    参阅全部 HLA DR 一抗
  • 描述
    小鼠单克隆抗体[GRB-1] to HLA DR (PerCP)
  • 偶联物PerCP. Ex: 482nm, Em: 675nm
  • 特异性Reacts with cells of the monocytic lineage, with myeloblasts and promyelocytes and cells of the B lymphocyte lineage. Does not react with polymorphonuclear leukocytes and platelets.
  • 经测试应用适用于: Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Stimulated Human B lymphocytes

  • 阳性对照
    • Normal EDTA-anticoagulated peripheral blood. Nalm-6 cell line.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 1% BSA, pH 7.2
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆单克隆
  • 克隆编号GRB-1
  • 同种型IgG2a
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab91333 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt
  • 应用说明Flow Cyt: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form an heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal miroenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading.
    • 序列相似性Belongs to the MHC class II family.
      Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
    • 翻译后修饰Ubiquitinated by MARCH1 or MARCH8 at Lys-244 leading to down-regulation of MHC class II. When associated with ubiquitination of the beta subunit of HLA-DR: HLA-DRB4 'Lys-254', the down-regulation of MHC class II may be highly effective.
    • 细胞定位Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus > trans-Golgi network membrane. Endosome membrane. Lysosome membrane. Late endosome membrane. The MHC class II complex transits through a number of intracellular compartments in the endocytic pathway until it reaches the cell membrane for antigen presentation.
    • Information by UniProt
    • 数据库链接
    • 别名
      • DR alpha chain antibody
      • DR alpha chain precursor antibody
      • DRA_HUMAN antibody
      • DRB1 antibody
      • DRB4 antibody
      • Histocompatibility antigen HLA DR alpha antibody
      • HLA class II histocompatibility antigen antibody
      • HLA class II histocompatibility antigen DR alpha chain antibody
      • HLA DR1B antibody
      • HLA DR3B antibody
      • HLA DRA antibody
      • HLA DRA1 antibody
      • HLA DRB1 antibody
      • HLA DRB3 antibody
      • HLA DRB4 antibody
      • HLA DRB5 antibody
      • HLA-DRA antibody
      • HLADR4B antibody
      • HLADRA1 antibody
      • HLADRB antibody
      • Major histocompatibility complex class II DR alpha antibody
      • Major histocompatibility complex class II DR beta 1 antibody
      • Major histocompatibility complex class II DR beta 3 antibody
      • Major histocompatibility complex class II DR beta 4 antibody
      • Major histocompatibility complex class II DR beta 5 antibody
      • MGC117330 antibody
      • MHC cell surface glycoprotein antibody
      • MHC class II antigen DRA antibody
      • MHC II antibody
      • MLRW antibody
      see all

    Anti-HLA DR antibody [GRB-1] (PerCP) 图像

    • Flow cytometry analysis of Human peripheral blood cells, staining HLA DR with ab91333.

      PBMCs were isolated using a Ficoll gradient and fixed using paraformaldehyde. The sample was incubated with the primary antibody (1/100 in 2% human serum, 0.5 mM EDTA in PBS) for 30 minutes at 4°C.

      Gating Strategy: FSC/SSC lymphocytes and then FSC-A/FSC-H singlets

      See Abreview

    Anti-HLA DR antibody [GRB-1] (PerCP) (ab91333)参考文献

    ab91333 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Flow Cytometry
    Sample Human Cell (Peripheral blood cells)
    Specification Peripheral blood cells
    Preparation Cell harvesting/tissue preparation method: PBMCs were isolated using a Ficoll gradient
    Sample buffer: Ficoll/PBS
    Fixation Paraformaldehyde
    Permeabilization No
    Gating Strategy FSC/SSC lymphocytes and then FSC-A/FSC-H singlets
    Username

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    Verified customer

    提交于 Sep 14 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"