• 产品名称Anti-HLA-DQA1抗体
    参阅全部 HLA-DQA1 一抗
  • 描述
    小鼠单克隆抗体to HLA-DQA1
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Recombinant Fragment
    预测可用于: Human, Chimpanzee, Cynomolgus Monkey, Rhesus monkey, Gorilla
  • 免疫原

    GST-tagged recombinant fragment: EDIVADHVAS CGVNLYQFYG PSGQYTHEFD GDEEFYVDLE RKETAWRWPE FSKFGGFDP QGALRNMAVA KHNLNIMIKR YNSTAATN, corresponding to amino acids 24-111 of Human HLA-DQA1 (NP_002113)



Our Abpromise guarantee covers the use of ab76562 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
  • 应用说明ELISA: Use at an assay dependent dilution.
    WB: 1/500 - 1/1000. Predicted molecular weight of the endogenous protein: 28 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accomodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form an heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal miroenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading.
    • 序列相似性Belongs to the MHC class II family.
      Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
    • 细胞定位Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus > trans-Golgi network membrane. Endosome membrane. Lysosome membrane. The MHC class II complex transits through a number of intracellular compartments in the endocytic pathway until it reaches the cell membrane for antigen presentation.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CD antibody
      • CELIAC1 antibody
      • DC 1 alpha chain antibody
      • DC alpha antibody
      • DC-1 alpha chain antibody
      • DC-alpha antibody
      • DC1, included antibody
      • DQ alpha 1 chain antibody
      • DQ-A1 antibody
      • DQ-DRW9 alpha chain antibody
      • DQA1_HUMAN antibody
      • FLJ27088 antibody
      • FLJ27328 antibody
      • Gluten-sensitive enteropathy (celiac disease) antibody
      • GSE antibody
      • HLA class II histocompatibility antigen antibody
      • HLA class II histocompatibility antigen, DQ alpha 1 chain antibody
      • HLA class II histocompatibility antigen, DQ(W3) alpha chain antibody
      • HLA-DCA antibody
      • HLA-DQA antibody
      • HLA-DQA1 antibody
      • HLA-DQA1 major histocompatibility complex, class II, DQ alpha 1 antibody
      • HLADC histocompatibility type antibody
      • Immune response antigens HIa, included antibody
      • leucocyte antigen DQA1 antibody
      • leukocyte antigen alpha chain antibody
      • LOC100133678 antibody
      • LOC100507686 antibody
      • LOC100509457 antibody
      • Major histocompatibility complex, class II, DQ alpha 1 antibody
      • MGC149527 antibody
      • MHC class II antigen antibody
      • MHC class II DQA1 antibody
      • MHC class II HLA-D alpha glycoprotein antibody
      • MHC class II HLA-DQ alpha 1 antibody
      • MHC class II surface glycoprotein antibody
      • MHC HLA-DQ alpha antibody
      • OTTHUMP00000029141 antibody
      • OTTHUMP00000176885 antibody
      • OTTHUMP00000178551 antibody
      • OTTHUMP00000178552 antibody
      • OTTHUMP00000233817 antibody
      see all

    Anti-HLA-DQA1 antibody 图像

    • Anti-HLA-DQA1 antibody (ab76562) at 1/500 dilution + immunogen (GST-tagged recombinant fragment) at 0.2 µg

      Goat Anti-Mouse IgG (H&L) HRP-conjugated secondary antibody at 1/2500 dilution

      Predicted band size : 28 kDa
      Observed band size : 36 kDa (why is the actual band size different from the predicted?)

    Anti-HLA-DQA1 antibody (ab76562)参考文献

    ab76562 has not yet been referenced specifically in any publications.

    Product Wall

    Here is a link to the fractionation protocol as discussed: I really think that enriching for the membrane fraction may help. I will also discuss with one of my colleagues.

    I am sorry for the delay in responding to you. I tried to call you yesterday and today but was unable to reach you, therefore I would prefer to send an email to ensure a speedy resolution. It is very disappointing that 4 products are not workin...

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