概述

  • 产品名称Anti-HLA DQ1 + HLA DQ3抗体[HL-37]
  • 描述
    小鼠单克隆抗体[HL-37] to HLA DQ1 + HLA DQ3
  • 特异性Reacts with polymorphic determinant on human HLA-DQ1 and HLA-DQ3 molecules (recognized epitope was found on isolated b chain of DQ1), but does not react with HLA-DQ2.
  • 经测试应用适用于: Flow Cyt, WB, ICC/IF, IPmore details
  • 种属反应性
    与反应: Human, Pig
  • 免疫原

    Tissue/ cell preparation (Human). Burkitt lymphoma cell line Raji.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 15mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 纯度>95% by SDS-PAGE
  • 纯化说明Purified from ascities by protein-A affinity chromatography.
  • 克隆单克隆
  • 克隆编号HL-37
  • 骨髓瘤unknown
  • 同种型IgG3
  • 轻链类型unknown
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab2600 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use a concentration of 5 µg/ml.
WB Use at an assay dependent concentration. Use under non reducing condition.
ICC/IF Use at an assay dependent concentration. PubMed: 20959457
IP Use at an assay dependent concentration.

Anti-HLA DQ1 + HLA DQ3 antibody [HL-37] 图像

  • HeLa cells were transfected with DQ. The cells were fixed in paraformaldehyde and either left unpermeabilized (-Tx) or were permeabilized with 0.2% Triton X-100 (+Tx). DQ-transfected cells were immunostained with ab2600. Nuclei were stained with DAPI.
  • HeLa cells were transfected with different combinations of a, ß, and Ii constructs as indicated. Lysates were first subjected to immunoprecipitation (IP) with ab2600 before Western blotting (WB) with anti-DQ and anti-Ii. H and L indicate the heavy and light chains of the antibodies used for the immunoprecipitation.

Anti-HLA DQ1 + HLA DQ3 antibody [HL-37] (ab2600)参考文献

This product has been referenced in:
  • van Lith M  et al. HLA-DP, HLA-DQ, and HLA-DR Have Different Requirements for Invariant Chain and HLA-DM. J Biol Chem 285:40800-8 (2010). ICC/IF, IP ; Human . Read more (PubMed: 20959457) »
  • Horejsi­ V  et al. Characterization of seven new monoclonal antibodies against human DR, DR + DP and DQ1 + DQ3 antigens. Tissue Antigens 28:288-97 (1986). Read more (PubMed: 2435017) »

See all 2 Publications for this product

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