概述
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产品名称Anti-HLA Class I抗体[YTH862.2]
参阅全部 HLA Class I 一抗 -
描述大鼠单克隆抗体[YTH862.2] to HLA Class I
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特异性ab33252 recognises a monomorphic determinant on the human HLA Class I heavy chain. We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
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经测试应用适用于: Functional Studies, Flow Cyt, IHC-Fr, IPmore details
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种属反应性与反应: Human
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免疫原
Tissue/ cell preparation: PHA activated human peripheral blood lymphocytes.
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表位ab33252 reacts with an epitope located in the alpha1 domain of HLA class I molecules.
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阳性对照
- Human peripheral blood lymphocytes
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常规说明
Fusion Partners: Spleen cells from immunised DA rats were fused with cells of the rat Y3 myeloma cell line.
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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存储溶液Preservative: 0.09% Sodium Azide
Constituents: PBS, pH 7.4 -
Concentration information loading... -
纯度Protein G purified
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纯化说明ab33252 was purified from tissue culture supernatant.
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克隆单克隆
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克隆编号YTH862.2
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同种型IgG2b
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研究领域
相关产品
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Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab33252 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Functional Studies | Use at an assay dependent concentration. This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays. | |
| Flow Cyt | 1/50 - 1/100. Use 10µl of the suggested working dilution to label 106 cells in 100µl. ab18536-Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
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| IHC-Fr | Use at an assay dependent concentration. | |
| IP | Use at an assay dependent concentration. |
靶标
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相关性HLA CLass I is involved in the presentation of foreign antigens to the immune system.
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细胞定位Plasma membrane
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数据库链接
- Entrez Gene: 3105 Human
- Entrez Gene: 3133 Human
- Entrez Gene: 3134 Human
- Entrez Gene: 3135 Human
- Omim: 142800 Human
- SwissProt: O15506 Human
- SwissProt: P01891 Human
- SwissProt: P01892 Human
see all -
别名
- DKFZp686P19218 antibody
- EA1.2 antibody
- EA2.1 antibody
see all
Anti-HLA Class I antibody [YTH862.2] 图像
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![Flow Cytometry - HLA Class I antibody [YTH862.2] (ab33252)](http://www.abcam.cn/ps/datasheet/Images/33/ab33252/FACS HLA class i.jpg)
Flow Cytometry - HLA Class I antibody [YTH862.2] (ab33252)FACS analysis of HLA class I expressing cells in human peripheral blood lymphocytes using ab33252.
Anti-HLA Class I antibody [YTH862.2] (ab33252)参考文献
ab33252 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"