Anti-HLA Class I抗体[YTH862.2] (ab33252)

Abreviews (3)Q&A (2)

概述

  • 产品名称
    Anti-HLA Class I抗体[YTH862.2]
    参阅全部 HLA Class I 一抗
  • 描述
    大鼠单克隆抗体[YTH862.2] to HLA Class I
  • 宿主
    Rat
  • 特异性
    ab33252 recognises a monomorphic determinant on the human HLA Class I heavy chain. We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • 经测试应用
    适用于: Functional Studies, Flow Cyt, IHC-Fr, IPmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Tissue/ cell preparation: PHA activated human peripheral blood lymphocytes.

  • 表位
    ab33252 reacts with an epitope located in the alpha1 domain of HLA class I molecules.
  • 阳性对照
    • Human peripheral blood lymphocytes
  • 常规说明

    Fusion Partners: Spleen cells from immunised DA rats were fused with cells of the rat Y3 myeloma cell line.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 纯度
    Protein G purified
  • 纯化说明
    ab33252 was purified from tissue culture supernatant.
  • 克隆
    单克隆
  • 克隆编号
    YTH862.2
  • 同种型
    IgG2b
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab33252 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent concentration. This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays.
Flow Cyt 1/50 - 1/100.

Use 10µl of the suggested working dilution to label 106 cells in 100µl.

 

 

 

ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

 
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

靶标

图片

  • Flow Cytometry - HLA Class I antibody [YTH862.2] (ab33252)
    Flow Cytometry - HLA Class I antibody [YTH862.2] (ab33252)
    FACS analysis of HLA class I expressing cells in human peripheral blood lymphocytes using ab33252.

文献

ab33252 has not yet been referenced specifically in any publications.

发表研究结果有使用 ab33252？请让我们知道，以便我们可以引用本数据表中的参考文章。

客户评价及客户问答

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-HLA Class I antibody [YTH862.2]

 Poor
Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Human xenograft in mouse)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer
Permeabilization
Yes - 0.1% Triton X
Specification
Human xenograft in mouse
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
Fixative
Formaldehyde
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Verified customer

提交于 Apr 18 2018

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-HLA Class I antibody [YTH862.2]

 Average
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Infarcted Mouse Heart injected /w or w/o human mon)
Antigen retrieval step
Heat mediated
Permeabilization
No
Specification
Infarcted Mouse Heart injected /w or w/o human mon
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Paraformaldehyde
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Abcam user community

Verified customer

提交于 Jul 20 2017

Testing discount code to try in ICC/IF.

DISCOUNT CODE: *********
Expiration date: ********

I am very pleased to hear you would like to accept our offer and test ab33252 in ICC/IF. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer...

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I am searching for an antibody to HLA Class I that can be used for staining human cells injected into mouse heart.

Thank you for your phone call. As we discussed, the best staining on mouse tissue will be obtained using a primary antibody raised in a species other than mouse in combination with a secondary antibody that has been pre-adsorbed to remove cross-reactiv...

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Immunohistochemistry (Frozen sections) abreview for Anti-HLA Class I antibody [YTH862.2]

 Average
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (skin)
Specification
skin
Fixative
Formaldehyde
Permeabilization
No
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 21°C
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Username

Abcam user community

Verified customer

提交于 Jun 17 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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