Anti-HLA Class I抗体[W6/32] - Low endotoxin, Azide free (ab23755)

概述

  • 产品名称Anti-HLA Class I抗体[W6/32] - Low endotoxin, Azide free
    参阅全部 HLA Class I 一抗
  • 描述
    小鼠单克隆抗体[W6/32] to HLA Class I - Low endotoxin, Azide free
  • 特异性The antibody recognises an antigenic determinant common to HLA A, B and C. In WB, it detects the heavy chain of MHC molecule. We have data to indicate that this antibody may not cross react with Rabbit. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • 经测试应用适用于: Flow Cyt, IP, WB, IHC-Fr, ELISA, ICC/IF, Functional Studiesmore details
  • 种属反应性
    与反应: Cow, Cat, Human
  • 免疫原

    Tissue/ cell preparation - Membrane of human tonsil cells.

  • 常规说明

    The antibody recognises virtually all nucleated human cells, it is a valuable reagent for analysing variations in HLA class I expression in different disease states e.g. liver disease, muscular dystrophy, inflammatory myopathy and other neuromuscular disorders. This antibody is also suitable as a positive control for HLA tissue typing and crossmatching.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液Preservative: None
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 纯度Protein G purified
  • Primary antibody说明The antibody recognises virtually all nucleated human cells, it is a valuable reagent for analysing variations in HLA class I expression in different disease states e.g. liver disease, muscular dystrophy, inflammatory myopathy and other neuromuscular disorders. This antibody is also suitable as a positive control for HLA tissue typing and crossmatching.
  • 克隆单克隆
  • 克隆编号W6/32
  • 同种型IgG2a
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab23755 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 1µg for 106 cells.

Paraformaldehyde or methanol fixed cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

IP Use at an assay dependent concentration.
WB 1/250. Use under non reducing condition. Detects a band of approximately 45 kDa (predicted molecular weight: 31 kDa).
IHC-Fr Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 µg/ml.
Functional Studies Use at an assay dependent concentration.

靶标

Anti-HLA Class I antibody [W6/32] - Low endotoxin, Azide free 图像

  • ICC/IF image of ab23755 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab23755, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Overlay histogram showing Jurkat cells stained with ab23755 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab23755, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with methanol (5 min) used under the same conditions.

    Please note that Abcam do not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Anti-HLA Class I antibody [W6/32] - Low endotoxin, Azide free (ab23755)参考文献

This product has been referenced in:
  • Martorelli D  et al. IGKV3 proteins as candidate "off-the-shelf" vaccines for kappa-light chain-restricted B-cell non-Hodgkin lymphomas. Clin Cancer Res 18:4080-91 (2012). Human . Read more (PubMed: 22705988) »
  • Park B  et al. The HCMV membrane glycoprotein US10 selectively targets HLA-G for degradation. J Exp Med 207:2033-41 (2010). WB ; Human . Read more (PubMed: 20713594) »

See all 5 Publications for this product

Product Wall

Application Flow Cytometry
Sample Human Cell (TE11, PBMC)
Permeabilization No
Gating Strategy HLA Class I positive living cells
Specification TE11, PBMC
Fixation None
Username

Abcam user community

Verified customer

提交于 Nov 09 2015

I have received a reply from the lab who confirm that we have tested only cell surface binding of this antibody (which was good), not intracellular binding.

Currently we do not have data regarding its reactivity with premature antigen. However...

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Thank you for contacting us.

To our knowledge our HLA Class I antibodies have not been tested to not detect HLA-G. I did find that our anti-HLA Class I [MEM-123] antibodies have the following specificity:

This antibody reacts with al...

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Thank you for your enquiry.

I am sorry to confirm that this antibody is not tested and covered by our 6 monthy guarantee for this particular application. For more details regarding our guarantee, please see the following page from our websit...

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The reactivity with bovine material is good. With regard to this product's cross reactivity with murine material please see the article referenced below:



Kievits F, ...

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Yes, we did offer that customer a discount to test the antibody in rat in IHC-P. We have a testing discount program where customers can purchase an antibody risk-free to try in their novel species and/or applicatio...

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Expiration date: *********

I am very pleased to hear you would like to accept our offer and test ab23755 in mouse tissue. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem th...

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Thank you for your response. Lysates from Jurkat or HeLa cells or from human tonsil tissue can be used as positive control. Currently, we have do not have HLA Class I over-expression cell lysate in our catalogue. It may be worth optimizing the working ...

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Thank you for your enquiry and your interest in our products. Anti-alpha tubulin (clone TU-01) can be used as loading control together with antibody HLA Class I (ab23755) . Currently, we have several forms of this antibody ie. unconjugated as well as c...

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I am very pleased to hear you would like to accept our offer and test ab23755 in IHC-P. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for IHC-P and include this code in the ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"