Anti-HLA Class I抗体[MEM-81] (ab3975)

概述

  • 产品名称Anti-HLA Class I抗体[MEM-81]
    参阅全部 HLA Class I 一抗
  • 描述
    小鼠单克隆抗体[MEM-81] to HLA Class I
  • 特异性The antibody reacts with human classical MHC Class I molecules in native cell-surface forms.
  • 经测试应用适用于: IHC-P, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Tissue/ cell preparation (Human). Leucocytes of a patient suffering with LGL type leukaemia.

  • 阳性对照
    • IHC-P: human spleen FFPE tissue sections

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 15mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 纯度>95% by SDS-PAGE
  • 纯化说明Purified from ascites by protein-A affinity chromatography.
  • 克隆单克隆
  • 克隆编号MEM-81
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab3975 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt Use a concentration of 1 µg/ml.

靶标

Anti-HLA Class I antibody [MEM-81] 图像

  • Overlay histogram showing Jurkat cells stained with ab3975 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab3975, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • IHC image of ab3975 staining in human spleen formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab3975, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-HLA Class I antibody [MEM-81] (ab3975)参考文献

ab3975 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your email. Ab2217 is isotype IgG3; ab9090 is isotype IgG1; and ab3975 is isotype IgG1. You may also find the isotype information on our online datasheets. If you have any additional questions, please contact us again.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"