小鼠单克隆抗体[MEM-147] to HLA Class I (Phycoerythrin)
Phycoerythrin. Ex: 488nm, Em: 575nm
recognises all human classical MHC Class I molecules in native
cell-surface forms (e.g. it recognizes native HLA-A2 in cytofluorometry and immunoprecipitation but not in Western blotting). MHC Class I molecules (MHC Class Ia) are expressed on the surface of all human cell types.
ab52451 is positive in Western blotting (non-reducing conditions) only with most HLA-B and HLA-C molecules, but not HLA-A.
PHA-activated peripheral blood lymphocytes.
Shipped at 4°C. Store at +4°C.
Preservative: 15mM Sodium Azide
Constituents: 0.2% BSA (high-grade protease free), PBS
Concentration information loading...
>95% by SDS-PAGE
ab52451 was purified by Protein G affinity crimatography prior to conjugation. After conjugation, it was purified by site exclusion chromatography.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 µg/ml.
ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
HLA CLass I is involved in the presentation of foreign antigens to the immune system.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"