Anti-HLA Class I抗体[MEM-147] (ab9090)

概述

  • 产品名称Anti-HLA Class I抗体[MEM-147]
    参阅全部 HLA Class I 一抗
  • 描述
    小鼠单克隆抗体[MEM-147] to HLA Class I
  • 特异性Reacts with all human classical MHC Class I molecules in native cell-surface forms. Positive in immunoblotting with many (not all) MHC Class I allelic forms (e.g. it recognizes native HLA-A2 in cytofluorometry and immunoprecipitation but not in Western blotting). Positive in Western blotting (non-reducing conditions) with most HLA-B and HLA-C molecules, but not HLA-A. Epitope specificity is very similar to classical antibody W6/32 (ab7855).
  • 经测试应用适用于: Flow Cyt, IP, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    PHA-activated peripheral blood lymphocytes

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 15mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 纯度>95% by SDS-PAGE
  • 克隆单克隆
  • 克隆编号MEM-147
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab9090 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IP Use at an assay dependent concentration.
WB 1/100. Use non-reducing conditions.

靶标

Anti-HLA Class I antibody [MEM-147] 图像

  • Anti-HLA Class I antibody [MEM-147] (ab9090) at 1/100 dilution + Human Ramos B cell line lysates (1% laurylmaltoside)

    Secondary
    Goat anti-mouse IgG (H+L)-HRP conjugate

    Performed under non-reducing conditions.
  • Overlay histogram showing Jurkat cells stained with ab9090 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9090, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 80% methanol (5 min) fixed Jurkat cells used under the same conditions.
    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Anti-HLA Class I antibody [MEM-147] (ab9090)参考文献

This product has been referenced in:
  • Tran TM  et al. The epitope recognized by pan-HLA class I-reactive monoclonal antibody W6/32 and its relationship to unusual stability of the HLA-B27/beta2-microglobulin complex. Immunogenetics 53:440-6 (2001). Read more (PubMed: 11685454) »

See 1 Publication for this product

Product Wall

Thank you for your enquiry. For this particular antibody, we unfortunately do not have this information. Our source for ab9090 has also said that they think that a pan MHC antibody that recognizes only the free heavy chains on the surface (where it is ...

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Thank you for your email. Ab2217 is isotype IgG3; ab9090 is isotype IgG1; and ab3975 is isotype IgG1. You may also find the isotype information on our online datasheets. If you have any additional questions, please contact us again.

The supplier gave me the following information regarding your question. If you have any additional questions, please contact us again. The antibody MEM-147 (ab9090) does not react with HLA-G1 overexpressed in transfectants. It can be used for IP ex...

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Interesting question, but I'm afraid that we are unable to offer any suggestions. Please let us know if you find a suitable target.

As far as we are aware, this antibody has not yet been tested in this application.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"