Anti-HLA Class I A3抗体[7g7h8] (ab86597)

概述

  • 产品名称Anti-HLA Class I A3抗体[7g7h8]
    参阅全部 HLA Class I A3 一抗
  • 描述
    小鼠单克隆抗体[7g7h8] to HLA Class I A3
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human HLA Class I A3.

  • 阳性对照
    • This antibody gave a positive signal in the following whole cell lysates: Jurkat; Raji; U937. Abcam recommends using 3% milk as a blocking agent.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度IgG fraction
  • 克隆单克隆
  • 克隆编号7g7h8
  • 骨髓瘤Sp2/0
  • 同种型IgG1
  • 轻链类型kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab86597 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).

Use 3% milk as a blocking agent.

靶标

Anti-HLA Class I A3 antibody [7g7h8] 图像

  • All lanes : Anti-HLA Class I A3 antibody [7g7h8] (ab86597) at 1 µg/ml

    Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 2 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
    Lane 3 : U937 (Human leukemic monocyte lymphoma cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 41 kDa
    Observed band size : 41 kDa


    Exposure time : 20 minutes

Anti-HLA Class I A3 antibody [7g7h8] (ab86597)参考文献

ab86597 has not yet been referenced specifically in any publications.

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Lab has confirmed that they have not determined the endotoxin level of antibody ab33640 and ab86597. This means we will not bwe able to confirm how the antibodies will perform in cell culture experiments.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"