Anti-HLA B27抗体[HLA-ABC-m3] (FITC) (ab23840)

概述

  • 产品名称Anti-HLA B27抗体[HLA-ABC-m3] (FITC)
    参阅全部 HLA B27 一抗
  • 描述
    小鼠单克隆抗体[HLA-ABC-m3] to HLA B27 (FITC)
  • 偶联物FITC. Ex: 493nm, Em: 528nm
  • 特异性ab23840 is a cytotoxic antibody which detects HLA B27 alloantigen.
  • 经测试应用适用于: Flow Cyt, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Immune complex precipitated from HLA B27 positive cell line (Bordin) by anti HLA-antibody and Staphylococcal Protein A.

  • 常规说明


    ab23840 reacts with the peripheral blood lymphocytes of 47/47 individuals conventionally typed as HLA B27 + and precipitates cell surface molecules of 43 and 12 kD, corresponding to the HLA Class 1 heavy chain and beta 2 microglobulin.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯化说明ab23840 is a purified IgG prepared by ion exchange chromatography.
  • Primary antibody说明ab23840 reacts with the peripheral blood lymphocytes of 47/47 individuals conventionally typed as HLA B27 + and precipitates cell surface molecules of 43 and 12 kD, corresponding to the HLA Class 1 heavy chain and beta 2 microglobulin.
  • 克隆单克隆
  • 克隆编号HLA-ABC-m3
  • 同种型IgG2a
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab23840 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 10µl for 106 cells. ab91362-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration. PubMed: 18776939

靶标

  • 相关性Involved in the presentation of foreign antigens to the immune system. HLA B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta 2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells.
  • 细胞定位Single-pass type 1 membrane protein
  • 数据库链接
  • 别名
    • HLAB antibody
    • Leukocyte antigen class I B antibody
    • Lymphocyte antigen antibody
    • Major histocompatibility complex class I B antibody
    • SPDA1 antibody
    see all

Anti-HLA B27 antibody [HLA-ABC-m3] (FITC) (ab23840)参考文献

This product has been referenced in:
  • de Jong MA  et al. TNF-alpha and TLR agonists increase susceptibility to HIV-1 transmission by human Langerhans cells ex vivo. J Clin Invest 118:3440-3452 (2008). ICC/IF ; Human . Read more (PubMed: 18776939) »
  • Orr K  et al. Utilization of commercial antisera and flow cytometry in HLA-B27 typing. Cytometry 18:17-20 (1994). Read more (PubMed: 8082482) »

See all 3 Publications for this product

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The number of smaples can we worked out from the information on the datasheet, 250ul of antibody is provided. The amountof tess in can do will very much depend on the dilution of antibody that you use, which may r...

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Thank you very much for sending the images. It really helps us understand the problem. I can see from the protocol sent that peripheral blood mononuclear cells were used for the ICC assay. It is a very mixed heterogeneous population, containing differe...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"