Anti-HLA B27抗体[HLA-ABC-m3] (FITC) (ab23840)

概述

  • 产品名称
    Anti-HLA B27抗体[HLA-ABC-m3] (FITC)
    参阅全部 HLA B27 一抗
  • 描述
    小鼠单克隆抗体[HLA-ABC-m3] to HLA B27 (FITC)
  • 偶联物
    FITC. Ex: 493nm, Em: 528nm
  • 特异性
    ab23840 is a cytotoxic antibody which detects HLA B27 alloantigen.
  • 经测试应用
    适用于: Flow Cyt, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Immune complex precipitated from HLA B27 positive cell line (Bordin) by anti HLA-antibody and Staphylococcal Protein A.

  • 常规说明


    ab23840 reacts with the peripheral blood lymphocytes of 47/47 individuals conventionally typed as HLA B27 + and precipitates cell surface molecules of 43 and 12 kD, corresponding to the HLA Class 1 heavy chain and beta 2 microglobulin.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯化说明
    ab23840 is a purified IgG prepared by ion exchange chromatography.
  • Primary antibody说明
    ab23840 reacts with the peripheral blood lymphocytes of 47/47 individuals conventionally typed as HLA B27 + and precipitates cell surface molecules of 43 and 12 kD, corresponding to the HLA Class 1 heavy chain and beta 2 microglobulin.
  • 克隆
    单克隆
  • 克隆编号
    HLA-ABC-m3
  • 同种型
    IgG2a
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab23840 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 10µl for 106 cells. ab91362-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration. PubMed: 18776939

靶标

  • 相关性
    Involved in the presentation of foreign antigens to the immune system. HLA B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta 2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells.
  • 细胞定位
    Single-pass type 1 membrane protein
  • 数据库链接
  • 别名
    • HLAB antibody
    • Leukocyte antigen class I B antibody
    • Lymphocyte antigen antibody
    • Major histocompatibility complex class I B antibody
    • SPDA1 antibody
    see all

文献

This product has been referenced in:
  • Chen LG  et al. Therapeutic targeting of liver cancer with a recombinant DNA vaccine containing the hemagglutinin-neuraminidase gene of Newcastle disease virus via apoptotic-dependent pathways. Oncol Lett 12:3344-3350 (2016). Flow Cyt . Read more (PubMed: 27900002) »
  • de Jong MA  et al. TNF-alpha and TLR agonists increase susceptibility to HIV-1 transmission by human Langerhans cells ex vivo. J Clin Invest 118:3440-3452 (2008). ICC/IF ; Human . Read more (PubMed: 18776939) »

See all 4 Publications for this product

客户评价及客户问答

Thank you for your enquiry.

The number of smaples can we worked out from the information on the datasheet, 250ul of antibody is provided. The amountof tess in can do will very much depend on the dilution of antibody that you use, which may r...

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Thank you very much for sending the images. It really helps us understand the problem. I can see from the protocol sent that peripheral blood mononuclear cells were used for the ICC assay. It is a very mixed heterogeneous population, containing differe...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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